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• <br />2455 Estand Way <br />Pleasant Hill, CA 94523 <br />(510) 686-3215 FAX 686-0399 <br />Date: October 31, 1995 <br />To: Mr. Ed Hamilton <br />McCampbell Analytical <br />110 2nd Avenue, #D7 <br />Pacheco, CA 94553 <br />From: Prepared by: <br />Phyllis Riboni-Hustead <br />Staff Scientist <br />Julianne C. Fegley <br />Laboratory Coordinator <br />Subject: Aquatic Toxicity Testing Results for Hazardous Waste Screening Testing <br />P.O.#verbal <br />SAMPLE MATRIX AND I.D.: One solid(001, 2, 3, and 4) sample #14048. Project No: <br />57449. Project Name/No: T.S. - W.S/ 5062. <br />TREATMENT DILUTIONS (mg/L): 250, 750 and Control run in soft carbon filtered tap <br />water mixed with deionized water of 40-48 mg/L hardness and in duplicate with 10 fish/6 L <br />tank and 20 fish/treatment. <br />TESTING PERIOD: Received 10/16/95; Tested 10/19/95 - 10/23/95. <br />BIOASSAY TEST(S): Fathead minnow (Pi7nephales promelw) 96 -hour static Hazardous <br />Waste Toxicity. <br />METHODS: BES's Hazardous waste aquatic toxicity test protocol is based on "Standard <br />Methods for the Examination of Water and Wastewater", 18th Edition, American Public <br />Health Association, 1992; California's Title 22 Code, Section #66261.24(a)(6); "Static Acute <br />Bioassay Procedures for Hazardous Waste Samples" (Polisini and Miner, 1988), California <br />Department of Fish and Game; and as certified by the State of California's Department of <br />Toxic Substance Control (CA. EPA). <br />SUMMARY: <br />Fathead minnow 96 -hour percent survival in Control was 90%. <br />Fathead minnow 96 -hour percent survival in the test concentrations was 100% for 250 mg/L, <br />100% for 500 mg/1, and 100% for 750 mg/L. <br />The 96 -hour LC50 = >750 mg(L. <br />The transcribed data sheets, statistics and chain -of -custody for these tests are enclosed. If <br />you have any questions concerning this report please contact Phyllis Riboni Hustead, Staff <br />Scientist, at the lab (510) 682-7200. <br />