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Lab. No. 3 4 8 S 5 8- Z <br /> Date Received:11 10 G 201 L- <br /> LABORATORY WORKSHEET: Date Reported: '11 l i 1 j 1 4 <br /> Bioassay Type <br /> Report To: �eMe�no/K2t G�OpYL• Static V Continuous Renewal <br /> • Screening V Detlnitive <br /> b�3 <br /> Sample Description S o e t cL 15 - OA <br /> Test Organism: Pimephales Promelas Source: Thomas Fish Farm Acclimatization: 'I G Days .2 0 deg.C (FI3 -1 ) <br /> Aquaria Volume: 10 liters) Aquaria Depth: 5 inch(es) No.Fish/Concentration: 10 Total Chlorine Residual: w Sample Conductivity: 2.*46 Nmhos/cm <br /> Organism Characteristics - Length(mm): Min: 3 3: mm Max: 4•S mm Avg: 41 mm Weigh(gm): Min:0,40 gm Max: o.5 4 gm Avg: ,so am <br /> Dilution water - Source: Soft Water Hardness- Initial: 4.O mg/l Final: 66 mo/I Alkaline- Initial: 30 mo/I Final: 4o mq/I <br /> Aeration: N/A Control Hardness Initial: 40 mg/I. Final: _%QmgA .Control Alkaline Initial: 3 o mgll .Final 3_mg/I.Control Conductivity .s umhos/cm <br /> Aeration Rate: N/A Aeration Duration: Tanks: 1 <br /> Bioassay Date Control Dilution <br /> Conditions Time 750 500 250 DUP 750 DUP 500 DUP 250 <br /> No. % No. % No. % No. % No. % No. % No. % <br /> 10 100 10 100 10 100 10 100 10 100 10 100 10 100 <br /> Organisms 10 1,00 110 10Q 30 'i o o, 10 100 <br /> 00O <br /> Surviving 1 <br /> 1 1 C) 10 10 0 110 100 10 100 1 in 100 10 100 10 loo 10-11110 10 100 10 100 O 1 <br /> 1 o O 10 1 1 o n 10 10 ' <br /> Dissolved �• _ <br /> Oxygen ,R, <br /> mg/L ro. R G. <br /> r, 6-q O <br /> Start °C �q, °C °C °C °C °C y , °C <br /> pH 24 Hr 20. °C °C . °C 2 . °C 4 °C zo o°C '+. ,0°C <br /> 48 Hr °C --, zo,2°C 2,°C 2.0.z°C 2.o Z C a C 1 2o. °C <br /> Temp 72 Hr °C .5 20. °C 2,0.0°C 0.0°C .o°C �. a.0, °C 2.D O'C <br /> 96 Hr 1 °C 4 zo. °C 2- °C a. .1 °C '+.4� 20.1 °C ao °C ao.1 °C <br /> Results-LCso= > -+S o mg/l Method of Calculations fJ i <br /> > 5 n mg/I 95%Confidence Limits -Ac <br /> %Survival N/A LC50 Method <br /> Toxic Units T.U. N/A <br /> Observation/Remarks Laboratory Supervisor <br />