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A Keith Kaufman, M.S. <br /> IConsultant in Environmental Biotechnology <br /> December 8, 1999 Serving Industry,Government Academia and the Legal Profession <br /> TO Mr Bill Little (Advanced GeoEnvironmental, Inc ) <br /> FROM A Keith Kaufman <br /> Subject Results of Physicochemical and Microbiological Analyses of Four (4) Soil <br /> ISamples (Curtiss Pontiac) <br /> Listed below are the results from the referenced analyses performed on four (4) soil samples <br /> collected on 1 1118-19199 and received on 11/24/99 After appropriate dilution, samples were <br /> plated onto Trypticase Soy Agar (TSA, for general microbial enumeration) and Minimal Salts <br /> Agar (MSA) supplemented with 2000 ppm or 5000 ppm gasoline as sole carbon substrate <br /> Media were prepared at ambient pH Cultures were incubated under aerobic conditions at <br /> room temperature for six (6) days prior to enumeration Physicochemical analyses were <br /> I performed utilizing the HACH Spectrophotometric System In accordance with manufacturer's <br /> specifications <br /> IRESULTS <br /> Ipace �QW4_5 MW4-10 MW7-5 MW7-i0 <br /> I pH 102 104 100 103 <br /> Nitrate-N (ppm) 11 0 so 100 80 <br /> Nitrate (ppm) 484 35 2 440 352 <br /> I Ammonia-N (ppm) 150 170 150 180 <br /> Ammonium (ppm) 19 5 22 1 19 5 234 <br /> Ortho-Phosphate (ppm) 141 140 138 138 <br /> IPotassium (ppm) 1680 155 0 1720 1590 <br /> % Moisture 160% 184% 145% 18 1 % <br /> Gen Micr Enum <br /> (CFU X 105) 0 16 96 008 076 <br /> ISel Micr Enum <br /> (CFU X 105) <br /> Gas (2000 ppm) ------- 040 (4 2%) -------- 004 (5 3%) <br /> Gas (5000 ppm) 001 (63%) -------- NO ------- <br /> • ND, Not detected at limit of 0 1 ppm 1 10 dilution <br /> CFU, Colony-forming Units (viable cells) <br /> I United States Office CFU, <br /> Office <br /> I 6243 Bristol Parkway 449 2nd Floor,#162 Tung-An Street,Ta-An District <br /> Cuiver City,CA 90230 Taipei Taiwan R.O C <br /> (310)822-9385 (310)377-9364[FAX] 886-2-378-3731 886-2-378-3732(FAX) <br />