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FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY Bqua"c <br /> Testing <br /> Lab No.: A l to�r I I vS-,-an l Laboratories <br /> Client/ID: CdcG PS w/ ?Aewwl <br /> TEST SUMMARY <br /> Species:Pimephalespromelas. Source: 7 ,,,o,� ^� <br /> Fish weight(gm): av: o•St ; min: d-Y7 ; max: _. Regulations: CCR Title 22. <br /> Reference Toxicant: SDS conducted monthly per source. Test Protocol: California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints: Survival at 96 hrs. <br /> Temperature: 20+/-2°C. Test type: Static. <br /> Aeration: none/minimum(>4.0 mg/l DO). Feeding:None. <br /> Number of replicates: 2. Number of fish per chamber: ICO <br /> Dilution water: Soft reconstituted water(40-48 mg/l CaCO3). Photoperiod: 16/8 hrs light/dark. <br /> TEST DATA <br /> INITIAL 24 Hr 48 Hr 72 Hr 96 Hr <br /> Date/Time: K-0-14 2t yffr -(v-m (o a g-1-i S"(4 l vio q o To Ito-" <br /> Analyst: / E #-4_ 1[— Z <br /> _ <br /> °C DO pH °C DO pH #D °C DO pH #D °C DO pH #D °C DO pH # D <br /> Control A &2 g-,,S -f 7 d 1 7.4, 7 Y U <br /> Control B 25,o d,+/ g ( 5, 3 ). 1 L 73 U <. 7 ) d /' �• <br /> 400 mg/l A 1p. S. 3 7. r Zoo7. 72. 0l0 1 S <br /> 400 mg/1 B fr, 2 .3 7 Z G lk'y 4 Z U o.' 74 17. 1 O .t <br /> 750 mg/1 A Q, Z �L ��� U lo•( O lo.o 7 6 <br /> 750 m 1 B �g Zo.u , 2X 7•) G .V .`� lis � , I O b. <br /> Comments: Extraction method: Mechanical shaking _�/. Dissolved Oxygen(DO)readings in mg/l 02. <br /> None(aqueous solution) <br /> Test Aeration: None__A6&. <br /> * Aerated_�. (Minimum needed to maintain DO>5.5 mg/L through narrow-bore glass tube at<100 bubbles per minute) <br /> CONTROL IF HIGH CONCENTRATION Total Number Dead <br /> Alkalinity Hardness Alkalinity Hardness Control 0 / �-0 <br /> Initial 3,7 mg/l CaCO, q 4 mg/1 CaCO; 3 mg/1 Caco, S 1) mg/1 CaCO, 400 mg/l O / 10 <br /> Final 3 mg/l CaCO, � mg/l CaCO, 33 mg/l Caw, S-1 mg/l CaCO; L 750 mg/l / Z <br /> RESULTS <br /> the checked ✓ result applies based on fish survival rates of this test; NA- notapplicable) <br /> ,✓ PASSED LC50>750 mg/1 (<40%dead in 750 mg/l conc.) <br /> 1-1. FAILED ,i40% dead in 750 mg/1 (close to passing-definitive test recommended) <br /> VA FAILED LC50<400 mg/1 (>60%dead in 400 mg/l conc.) <br />