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FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY aquatic <br /> Testing <br /> Lab No.: A i l oqu o r_rry3 Laboratories <br /> Client/ID: C B Sc PS GMD.I <br /> TEST SUMMARY <br /> Species:Pimephales promelas. Source: /T/ u,,as <br /> Fish weight(gm): av: D.S I ; min: c%y 7 ; max: 0. _. Regulations: CCR Title 22. <br /> Reference Toxicant: SDS conducted monthly per source. Test Protocol: California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints: Survival at 96 hrs. <br /> Temperature: 20+/-2°C. Test type: Static. <br /> Aeration: none/minimum(>4.0 mg/l DO). Feeding: None. <br /> Number of replicates: 2. Number of fish per chamber: <br /> Dilution water: Soft reconstituted water(40-48 mg/l CaCO3). Photoperiod: 16/8 hrs light/dark. <br /> TEST DATA <br /> INITIA� 24 Hr 48 Hr 72 Hr 96 Hr <br /> Date/Time: <br /> Analyst: <br /> Ji °C DO I pH °C I DO I pH #D °C I DO I pH 1 #D °C I DO I pH I #D °C I DO I pH # D <br /> Control A g,,S X d 07. C I I -(i 74 (-) ?.,)17. 117, <br /> Control B o a•y 7 % < ( S. 3 3 ?o. O <br /> 400 mg/1 A 8. 7 7 Q ZOO) v 4 Zo.l7.1 d <br /> 400 mg/l B 0 v .� Z S:K .2 to jr <br /> 750 mg/1 A ,� �,3 7_>: o.t �.) y,t d ZUI/ S .2 10, t 6. t 7 3 6 l0 3 1 3 7 5 0 <br /> 750 m 1 B (t f t, Y 17.9 1o•r �. O �k•1 V-�► 2 20,0 �i• 7 0 L. i.K O <br /> Comments: Extraction method: Mechanical shaking _�/. Dissolved Oxygen(DO)readings in mg/l 02. <br /> None(aqueous solution) <br /> Test Aeration: None_,n6d_. <br /> * Aerated ✓ . (Minimum needed to maintain DO>5.5 mg/L through narrow-bore glass tube at<100 bubbles per minute) <br /> �-CONTROL IF HIGH CONCENTRATION Total Number Dead <br /> Alkalinity Hardness Alkalinity Hardness Control V / 0 <br /> Initla)i 3 7 mg/1 CaCO, mg/1 CaCO, mg/l CaCo, mg/1 CaCO, 400 mg/l / 10 <br /> Final �) mg/l CaCO, mg/,CaCO, 3 mg/1 Caco, ro mg/l CaCO3 750 mg/l 1 0 <br /> RESULTS <br /> the checked -/ result applies based on fish survival rates of this test; NA- notapplicable) <br /> PASSED LC50>750 mg/1 (<40%dead in 750 mg/l cone.) <br /> FAILED 2-40%dead in 750 mg/1 (close to passing-definitive test recommended) <br /> FAILED LC50<400 mg/I (>60%dead in 400 mg/l cone.) <br />