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3 tha root ball in burlap and secure with wire. Dampen burlap with water, as necessary, to keep root ball wet; d. The elderberry shrub should be plantad in a water retention basin 40 feet by 40 feet in size (1600 square feet) . After removing the burlap and wire (if any) , plant the root ball so it is level with the existing ground. Compact the soil sufficiently so that settlement does not occur. live seedling elderberry shrubs and associated native vegetation (see number 4 below) should be planted in each of the basins; e. Saturate soil with water. Do not use fertilizers or other supplements or paint the tips of stems with pruning substance as the effects of these compounds on the beetle are unknown; f. Monitor to ascertain if additional watering is necessary: 1. if sandy, well-drained soil, plants may need to ba watered weekly or possibly twice monthly; 2. if clay, poorly-drained soil, it may not be necessary to water after the initial saturation. A drip watering system and timer would be ideal. However, in situations where ' this is not possible, a water truck or other apparatus may be used. j°�'�_^ Sixteen hundred (1600) square foet should be provided for each avoided 7o-4t! elderberry shrub, each transplanted elderberry shrub, GL and every five adF.w-�b-� transplanted elderberry shrubs and associated native plants (sea sections 3 and 4 balow) . The mitigation area should be designated as habitat for the valley elderberry longhorn beetle in perpetuity. The mitigation area should isolation of the beetl be designated to prevent e population from ether populations located in adjacent areas. A conservation easement or fee title for the mitigation area should be given to a resource agency or appropriate private organization. The Service should be provided with a map and written details specifically identifying the mitigation area prior to the initiation of the mitigation program. The applicant should receiva written approval from the Service that the mitigation area is acceptable prior to initiation of the i mitigation program. 3, procedure to plant additio ln� stems.--Each stem 1.0 inches or greater in diameter that is moved or destroyed will be replaced with seedling elderberry plants in the mitigation area using a ratio from 2:1 to 5:1. This replacement requirement applies even if the elderberry shrub is transplanted. Replacement stock should be obtained fromlocal sources. The approval of the Service should be obtained prior to initiating the compensation program. The ratio is determined as follows: Ratio of 2:1 Elderberry shrubs with stem diameters 1.0 inch or greatar and there arc no adult emergence holes. Ratio of 3:1 Elderberry shrubs with stem diameters 1.0 inch or greater. Beetles atc present as evidenced by emergence holes, and occur in 50 percent or Less of tho rhrubs. i b�'d LG'Ea WdyZ:7 Esst 'SZ atW vztGnLSTG :01 30Idd0 OH 010dS SPL-lSn:W0ad