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I(ennedy/Jenks Consultants <br /> Appendix B: Standard Operating Guideline <br /> Crop Biomass and Tissue Sampling <br /> I ! <br /> I Introduction <br /> This guideline describes the equipment and procedures that are used by Kennedy/Jenks <br /> Consultants personnel for sampling crop biomass and tissue sampling. <br /> 11 <br /> Equipment <br /> • Hand clippers or nursery shears <br /> • 1-gallon size zip-lock plastic bags and labeling pen <br /> • Insulated sample storage and shipping container <br /> Typical Procedure <br /> 1. This procedure should be completed for each harvest of each sample plot that is part of a Feld <br /> experiment. <br /> Biomass sampling. <br /> . 1 <br /> 2. Establish plot dimensions for the harvest(this is used to convert to yield per acre). <br /> 3. For machine harvest, the borders should be harvested first. Then the harvested the biomass <br /> from the plot. <br /> 4. If the biomass cannot be weighed immediately, collect tissue samples (see below) before <br /> weighing. <br /> 5. If the biomass is greater than 1,000 pounds, a truck scale may be used to weigh the tare and <br /> the loaded weight. For smaller harvest weights use a platform scale with 250 or 500 pound <br /> capacity. <br /> 6. The difference between the tare and the loaded weight is the biomass wet weight. <br /> Tissue sampling. <br /> 7. Collect a composite sample from the biomass. The zip-lock bag should be at least half full. <br /> Label and close the bag. Squeeze excess air from the bag while closing. <br /> 8. Place samples in an insulated container with blue ice and ship to an agricultural laboratory <br /> under chain of custody. <br /> 9. Be sure to include percentage moisture as one analyte. This is used to convert harvest biomass <br /> to dry weight. <br /> Crop Uptake and Assimilative Capacity Work Plan 1 <br /> Woodbridge Winery, Acampo, California <br /> fi g;tiaymupletlrmnpblOTOJOff].05 wmtlb,itlpe109�epalskrap vgske_msinul yep xk pMlµpeMkesbpp-0.tloc <br /> l <br />