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)6 AWnbWjk#JapF Wuxi Zhushi Sterilization Factory <br />)Mt -A- File Number SZLF/ST01_05-02 1 Version/revision number B/1 <br />fZ AT( I J Certification of EO Gas Sterilization <br />;PrP Customer <br />MW Approved by: <br />Approved 411% R M <br />2M. col <br />Suzhou Lengfeng Medical Instruments Co.,Ltd <br />leu PVP Productcodes <br />R'P p I Producttname <br />42-5 Lot No <br />/ <br />i] <br />KWADRON NEEDLES <br />0101,011AO120,0219, 0225.030ZUT0303.0304,0306,0311UT,0312,03128,03110T,0320,0320, <br />033018,0402,04078,0410,0510,0515, 05150, 0523, 0525, 0530, 06D6, 0606&0610,06106,0613, <br />0618, 0618& 0619, 0701, 0205, 0709, 07098, 07M 0717B, 0720, 07208, 0801, 0B01B4 0002, 0B1L <br />0815,0020, 00220, 0822PT, 0826, 090617; 0909, 0915t 0915AT5, 0915MZ4 09151614 0920UT, 0925, <br />1005, IODSH, 1010, <br />093�110BIIT,51109,119B,11136,1115,S11B'112OUT,1121, 112111P2TZ,, 11NUT 1123,1127, 1205, <br />1202, 1210, 1212, 1215U& 1219,1223,1225% U26, 1230,1231t 0108, 0110. 0111, 0115, 0117 -SL <br />0128, 0130, 0201, 0203s 022, 0226, 0229, 0305, 0306, 031$ 0322, 0325, 0406, 0416, D42, 0428, <br />0506, 0SUB, 052, 0515, 0516, 0520,0521,0522,0529.0530,0603,0600, 0612, 0617, 0626,0622UA. <br />070L 0202.0703, 0706,0707, 0710, 0718, 0219, 02204 072Z, 0723, 0726, 0230, 0807, 0310, 0817, <br />081& 0901,0902, 0904, 0905, 0906, 0914, 0915, 0916, 0917, 0921, 0923, 092641006,1007,1000. <br />1009,1010,1011,1012,1013,1017,1021,1022,102& 1024,1030,1104.1109,1111,1112,1113, <br />111M, 112% 1121, 1123, IMP 1130, 1201,1204MiS 1205, 1207o 1298, 1210T6L 1211, 1215. <br />MOM 1211M 1221, 1222, 12A 1225, 1220,1231,F0726 <br />TTom�+ lY <br />Sterilization mode <br />fiTgQ. <br />bd <br />Chamber NO. <br />`}Y,y, <br />EO y J` EO -Gas <br />2# <br />TM00 Sterilization parameters <br />R51! Validated <br />tifAg Tolerance <br />SRF Actual <br />fIRAZA Preconditioning Temperature (°C) <br />50 <br />±4 <br />50.5-53:5 <br />T511MA Preconditioning RH (%RH) <br />55 <br />±10 <br />49-55 <br />YMttt(Al Preconditioning Time (min) <br />360 <br />±20 <br />350 <br />7AW? Initial Evacuation Vacuum (]tpa) <br />-25 <br />±2 <br />-25.0 <br />19ARtful Keep Pressure (min) <br />20 <br />±10 <br />15 <br />XMP0AAR Weight of EO Injection(kg/25M3) <br />15 <br />-1-1.25 <br />15 <br />7F i3F1171j%t Rate of EO Injection (Icpa/min) <br />0.5 <br />±0.2 <br />0.50 <br />Tci'i'f*Ajx EO -Concentration (mg/L) <br />600 <br />±50 <br />600 <br />E09IAA BJJ91 EO Injection Pressure Changed(kpa) <br />>25 <br />// <br />5:25.013:8.0 <br />41FM Qt(al Exposure Time (min) <br />720 <br />±20 <br />720 <br />kffiffi& Sterilization Temperature (0C) <br />52 <br />±3 <br />50.5-53.5 <br />TCN &A Chamber RH of Sterilization (Yo RH) <br />55 <br />±10 <br />49,5-55.5 <br />A'MM Post Evacuation Vacuum (kpa) <br />-20 <br />±2 <br />-20.5 <br />#1;'L&ft The Number of Ventilator (times) <br />6 <br />// <br />6 <br />MrTRAE Aeration Temperature (0C) <br />42 <br />±5 <br />38.540.5 <br />f07#ratfa7 Aeration Time (day) <br />7 <br />// <br />7 <br />WLOI#4' Strain <br />BocillusPtrophoeusATCC9372 <br />3r)fg!"NominalPopulation(cfu/unit) <br />1.7x106 <br />BI Lot N0. <br />200825 <br />D fft D -Value (min) <br />3.0 <br />tri -ml Culture Temperat <br />3600 <br />)niful <br />Culture time(days) <br />7 <br />inrP HM Culture Date <br />2019-2020 <br />i rPHI iljCulture time(day <br />1st <br />2nd <br />3rd <br />4d1 <br />Sul 6th <br />7`11A,"WHiR <br />Observed Item <br />IMMIR Positive control <br />+ <br />+ <br />+ <br />+ <br />+ + <br />+ <br />�lJ3eT)fil3c li l $u` Bl test of sterility <br />❑o($ conformity <br />Wk' Conclusion: <br />.r.l�unA1 7'GI 17)(' F1069*f-f�)f2ic'i -, /'�° 7 (4T0 <br />The sterilization cycles were performed as per the validated param gfi t{ n viations. <br />The Sterile Assurance Level (SAL) of 106 is assured.' <br />1Y , <br />