Laserfiche WebLink
A I D S INFECTTCN CCNTRCL PRACTICES <br />1. Extraordinary care must be taken to avoid accidental wounds from sharp Instru- <br />ments (i.e., needles, syringes) contaminated with potentially infectious <br />material and to avoid contact of open skin lesions with material from, AIDS <br />patients. <br />2. Disposable .syringes and needles are preferred. *Cnly needle -locking syringes <br />or one-piece needle -syringe units should be used to aspirate fluids from <br />patients, so that collected fluid can be safely di.scharged through the needle, <br />if desired. <br />3. Glo-/es should be worn when handling blood specimens, blood -soiled Items, body <br />fluids, excretions, and secretions, as well as surfaces, materials, and objects <br />exposed to them. <br />4. Hands should be washed after removin,,C, gowns and gloves and before leaving the <br />0 <br />rooms of known or suspected AIDS patients. Hands should also be washed <br />thoroughly and immediately if they become contaminated with blood. <br />5. Blood and other specimens should be labeled prominently with a special warning <br />"AIDS Precautions." If the outside of the specimen container is visibly con- <br />taminated wtth blood, it should be cleaned with a disinfectant such as 1:10 <br />dilution of 5.25 per cent sodium hypochlorite (household bleach) with water. <br />All blood specimens should be placed in a second container, such as a bag, for <br />transport. The container or bag should be examined carefully for leaks or <br />cracks. <br />6. Mechanical pipetting devices should be used for the manipulation of all liquids <br />C� <br />in the laboratory. Mouth pipetting should not be allowed. <br />7. Laboratory coats, gowns, or uniforms should be worn while working with potentially <br />infectious materi.als and should be discarded appropriately before leaving the <br />laboratory. <br />8. All procedures and manipulations of potentially i.nfectious material should be per- <br />formed carefully to minimize the creation of droplets and aerosols. <br />9. Biological safety cabinets and other primary containment devices (e.g., centri- <br />fuge safety cups) are advised whenever procedures are conducted that have a high <br />potential for creat;.n- aerosols or infectious droplets. These include centri.- <br />fuging, blending, sonicating, vigorous mixing, and harvesting i.nfecti.ous tissue <br />from animals or embryonated eggs. <br />Fluorescent activated cell sorters generate droplets that could potentially <br />result in infectious aerosols. Translucent plastic '0 shielding between the droplet - <br />C, <br />collectin-, area and the equipment operator should be used to reduce the presently <br />uncertain magnitude of this risk. Primary containment devices are also used in <br />handling materials that might contain concentrated infectous agents or organisms <br />in greater quantities than expected in clinical specimens. <br />10. Laboratory work surfaces should be decontaminated with <br />th a disinfectant, such as <br />sodium hypochlorite solution, following any spill of potentially infectious material <br />C� <br />and at the completion of work activities. <br />11. All potentially contaminated materials used in laboratory tests should be decon- <br />taminated, preferably by autoclaving, before disposal or reprocessing. <br />12. All personnel should wash their hands following completion of laboratory activities, <br />removal of protective clothing, and before leaving the laboratory. <br />continued on next page---------- <br />