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Kendle, John - SAIC <br />From: Wagenman, Lois - SJMC <br />Sent: Friday, July 08, 20113:28 PM <br />To: Shepherd, Gloria - SJMC <br />Cc: Newman, Paula - SJMQ Kendle, John - SJMC <br />Subject: Failed biological tests on sterilizer in N comer lot of SJMC <br />Gloria, <br />Per your request, here are the various scenarios of the past 3 biological tests performed <br />on the steam sterilizer <br />used for biohazard waste in the North Corner Lot of SJMC. <br />Date of test: 7-1-2011 performed by: Larry <br />Lot # 2140 expiration date: 2/2012 <br />Positive control tube: positive (this is the expected result) <br />Test tube #1: negative in 24 hours <br />Test tube #2: postive in 24 hours (this is a failure) <br />Date of test: 7/5/2011 performed by: Melvin Tye <br />Lot # 2140 expiration date: 2/2012 <br />Positive control tube: positive <br />Test tube #1: negative in 3 days (prelim report only, we keep tubes for 7 days total) <br />Test Tube #2: negative in 3 days—still pending <br />So far, this biological test is performing as expected... final report will be sent out on <br />7/12/2011 <br />Date of test: 7/7/2011 performed by: Larry <br />Lot# 2140 expiration date: 2/2012 <br />Positive control tube: negative at 15 hrs <br />Test tube #1: positive at 15 hrs (failed test) <br />Test tube #2: positive at 15 hrs (failed test) <br />Gram stains performed on all positive tubes. They match the same organism as the control <br />tube. <br />The problems seem variable with the biological tests, so it is hard to determine whether <br />it is <br />technique or there is something wrong with the sterilizer. It would probably be a good <br />idea <br />to get it checked out and serviced. <br />Lois Wagenman <br />Microbiology Supervisor <br />St Joseph's Medical Center <br />HealthCare Clinical Lab <br />(209) 461-6819 <br />Lois.Wagenman@CHW.edu <br />11 <br />