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J' <br /> B. Biological indicator testing is done once per week with the first load of biological waste of the day. <br /> 1. Place culture in a small box, tape box so that culture does not fall out. <br /> 2. Place Biological indicator test packs in autoclave bag with red bag trash and tie off the bag. <br /> l <br /> 3. Pressure gauge on San-/-Pak/sterilizer must be checked and recorded before proceeding with the <br /> process. Pressure gauge reading should be above 72psi. San-I-Pak sterilizer recordings of <br /> temperature and pressure will be stored in EVS and kept for a minimum of three years. <br /> 4. At the completion of sterilization cycle, the biological indicator test indicator is taken to the in hospital <br /> Laboratory for incubation. Results are recorded in a 3MTM log book, which is located in the ETCH <br /> Laboratory. The log is kept for three years. <br /> 5. Incubation time for control test is 3 to 5 hours before reading and documented. <br /> 6. Control has to be kept for 24 hours after the test is completed for visual validation. <br /> C. Listing of specific materials needed for procedure: <br /> E <br /> 1. Control sample (unsterilized indicator) <br /> 2. Actual biological indicator(placed in sterilizer) <br /> 3. 3M Attest TM Rapid Biological indicator log book <br /> 4. 3M Attest TM Rapid Biological indicator incubator <br /> i <br /> A. Sources <br /> f <br /> 1. National Standards and Recommended Practices for Sterilization.Association for the Advancement <br /> of Medical Instrumentation,Arlington,Virginia,Vol 1_.1-1.2,1995. <br /> 2. CDC Control and Prevention, GuideforDisinfectionand Sterilization in HealthCare'—Monitoring. <br /> Last retrieved 1/17/17 from: htp://www.cdc.aov/hict)ac/disinfection sterilization/ <br /> 13 llsterilizingi2ractices.htmi <br /> 3. Waste Management Healthcare Solutions, Inc. <br /> 19966 Don Lee Place Ste. C <br /> Escondido, CA 92029 <br /> B. Author: Unknown <br /> 1. Reviewed by Dawn Kent, Manager Environmental Services 01/2017 <br /> C. Related policies and procedures: <br /> 1. STCH PolicyStat Portal Environmental Services—> Disposition of Trash Procedure (ES.20.00.00) <br /> 2. Refer to EVS policies on PolicyStat <br /> ATTAUCHMENTS <br /> A. None <br /> All revision dates: 2/10/2017,4/1/2013,6/1/2012,4/10/2012,4/20/2010: <br /> Aft ffifiihti� No Attachments <br /> Biological Testing of Autoclave/Sterilizer(for Biological Waste),ES-81-35-05.Retrieved 12/21/2018.Official copy at Page 2 of 3 <br /> http://sh-stch.policystat.com/policy/3185906/.Copyright 0 2018 Sutter Tracy Community Hospital <br />