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into the sanitary sewer. 3. Human Pathological and Tissue Cultures. a. Human pathological waste composed of tissues, organs, and body parts are considered biohazardous waste due to their possibilities of unknown pathogen in the patients or corpse. b. For aesthetic, recognizable body parts must not be disposed of in the normal waste stream. These types of wastes must be incinerated. Please call the Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine for more information. c. Cells or tissue cultures whether infected or not, must be autoclaved or chemically treated before disposal into a sanitary sewer. Cells or Tissue culture Chemical Treatment • Pour spent cells or tissue culture medium whether infectious or not, into a beaker or other vessel. • Add spoonful of hypochlorite granules or equal volume of chlorine (0.9%) to the discarded cells or tissue culture medium. • Mix well and hold overnight. • Stir, carefully pour supernant down sink and run copious amounts of water to flush. • Precipitant must be deposited into a biohazardous bag and autoclaved. • Place used beaker or other vessels to be autoclaved. d. For preserved specimen disposal, please see "preserved specimen policy". 4. Contaminated Biological Sharps a. All discard biological sharps (including but not limited to hypodermic needles, syringes, Pasteur pipettes, broken glassware, broken plasticware, lancets, glass slides, cover slips, plastic pipettes, pipette tips or any other material that is able to puncture a plastic trash bag) must be placed into impervious, rigid, puncture - resistant containers to avoid injury. These sharp containers must be located as close to the usage site as possible. Clipping, recapping, bending, manipulating, and breaking of needles are prohibited since this may result in the production of infectious aerosol and personal injury. Red sharps containers must be used solely for biological contaminated sharps. Opaque sharps containers is suggested for Radioactive contaminated sharps. Yellow sharps containers is suggested for exclusively chemically contaminated sharps. b. Sharps must be treated to eliminate the disease potential. Both incineration and steam sterilization provide effective treatment for contaminated sharps. Chemical decontamination maybe used. Chemical decontamination maybe used, with the approval of the Biological Safety Officer. C. Glass and Plastic sharps fileWCADocuments and Settings\OwnerNy Documents\P&P\Infection Control 2005\Bioha... 5/5/2005