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Lab Safety Lab Safety —Hood ventilation systems are best designed to have an airflow of —While performing work In the hood,the sliding not less than 60 feet per minute(fpm)and not more than 120 sash should be kept at the height designated fpm across the fate of the hood to provide the minimum face velocity required a (usually 100 fpm) —A mark should be placed on the hood so the sash can be drawn to —Work should be performed as deeply within the fume(rood as possible j a point where 100 fpm can be achieved �:�., • Equipment,reagents,and&ssware should be s —Avoid creation of strong cross drafts(100 fpm)caused by open placed as far back in the hood as is practical doors and windows,air conditioning and/or heating vents,or without blocking the rear baffle —ONLY ITEMS NECESSARY TO PERFORM THE personnel movements PRESENT EXPERIMENT SHOULD 11E IN THE a • Drafts will pull contaminants from the hood and Into the laboratory HOOD —When not in use,the sash of the hood should be kept closed •The more equipment In the hood,the greater the air turbulence and the chance for gaseous escape Into the lab i • r i I Lab Safety Lab Safety —When Instrumentation Isutilized for aproms Inside a Glassware hood,all Instruments should be elevated a minimum of two Inches from the hood base to facilitate proper ale _ — Although gloss vessels are frequently used In khx movement vacuum oPeradats,evacuated glass vessels —The purpose and function of a hood Is NOT to store collapse vicle n1%either spontaneously from strain chemicals or unused Items or from an accidental blow — Hoods shag not be used as a means of disposing of tock a Therefore,pressure and vxwm operations in elms Irritatingchemipis,but only asameansofremovingsmall vesselsshwid becooducted behind adequate jf y quantities of vapor which might escape during laboratory 'Ste, shlewlns - experiments !- — It is advisable to check for flaws won as star cracks, —Always look to assure fan motor power switch Is in the scratches and etching marks each time a vacuum d is apparatus use 'ON"position before working in the hood app —An emergency — Only round•bottomed or thkk-ailed(pyrntj B ntry Plan should be In the event c evacuated reaction vessels speciRcally designed for r . ventilation failure or other unexpected occurrence such as operations at reduced pressure should be used rare or explosion In the hood Lab Safety Lab Safety • Vacuums —The critical factors In vacuum pump selection are: —Vacuum pumps are used in the lab to Application the Pump will be used on remove air and other vapors from a vessel Nature of the sample(air,chemical,meisttxe) ora manifold size of the sample ls) —The most common usages are on rotary —When using a vacuum pump on a rotary evaporator,a dry evaporators,drying manifolds,centrifugal _ ke alcohol skrrrycold trap or a refrigerated trap Is '- recommended concentrators("speedvac"),acrylamide gel pCold Trap should be used in One with the pump when dryers,freeze dryers,vacuum ovens,tissue higher vapor loads from drying samples will occur culture filter flasks and aspirators, —vacuum um can um vapors from alr,water to toxic desiccators filtration apparatus and p pump 'syh flher/degassing apparatus and corrosive materials like Methylene chlorlde " `B 15