Laserfiche WebLink
T vaw a a.va aiaav/1�. LML—L— ;1 1111 ft V.4 . <br /> Oxygen Release Compound,ORC® <br /> Uses in Chlorinated Co-Metabolism <br /> One of the most promising applications for ORC is in the support of various co-metabolic <br /> processes In co-metabolism, certain enzymes produced and extruded by microorganisms, <br /> degrade chlorinated hydrocarbons. Unfortunately, some of the chlorinated hydrocarbon <br /> degradation products, such as epoxides, are toxic to the microorganisms and they must be <br /> constantly be "stoked" with substrates to maintain populations that allow the process to <br /> continue <br /> Step <br /> MMO (enzyme) <br /> Methane ----------------------------> Methanol CH30H ---------------------------> CQz <br /> Qxygen, NADH <br /> to <br /> MMO (enzyme) <br /> TCE ----------------------------------> Epoxide (toxic to MMO bacteria)------ ---> C- <br /> Q2 <br /> Qxygen, NADH <br /> Oxygen is Required in the Degradation of Methane to CO2 (Step 1), a reaction <br /> mediated by the enzyme Methane Monooxygenase (MMO). This same enzyme can <br /> also independently degrade chlorinated hydrocarbons in a process that also <br /> requires oxygen (Step 2). <br /> Recent advances in microbial selection and strain development have opened up the possibilities <br /> for bioremediation via these more rapid and efficient co-metabolic processes.Among these are <br /> the development of microorganisms that elicit the degratory enzymes more easily and in greater <br /> quantity. Also, more importantly, there is promise that epoxide resistant forms may be able to <br /> thrive rather than succumb in the presence of these toxic by-products <br /> Co-metabolism may be the reason reductions in PCE and TCE were observed with ORC in <br /> Iaboratory experiments carried out by Retec. The objective of the experiment was to see if <br /> ORC could inhibit vinyl chloride formation. In the course of this study the levels of TCE and <br /> PCE introduced into the culture environment were both reduced by two thirds in two months - <br /> relative to the control Furthermore, the authors of the study did not rule out direct chemical <br /> oxidation as a full or partial mechanism for the observed results <br /> JF3EGENE61 --------------------------------------------------------------------------- <br /> Bioremediation Products 27130A Paseo Espada, Ste 1407, San Juan Capistrano, CA 92675 <br /> Phone 714-443-3136 Fax 714-443-3140 <br />