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Lab. No. f - 1 <br /> Date Received. O 4 9 <br /> LABORATORY WORKSHEET Date Reported: o 9 04 1 <br /> Bioassay Type <br /> Report To: i1VI f0 L�tiQ,lY1, Static V Continuous Renewal <br /> f3 p f Screening V Definitive <br /> 1 6310, <br /> Sample Description UC1Wd, - a N S F - B-T L: - 16 1304;V3 - g <br /> Test Organism: Pimeohales Promelas (Qr- Source: Thomas Fish Farm Acclimatization: 19 Days 20 deg.C (F B - 1 ) <br /> Aquaria Volume: 10 liter(s) Aquaria Depth: 5 inch(es) No. Fish/Concentration: 10 Total Chlorine Residual: N Sample Conductivity: a Pmhos/cm <br /> Organism Characteristics - Length(mm): Min: 30 mm Max: SFO mm Avg: _ _ � mm '-.'Veigh(gm): Min: o.44gm Max. CI-64 gm Avg: O.5 gm <br /> Dilution water - Source: Soft Water Hardness- Initial: 40 mg/I Final: S� mq/1 Alkaline- Initial: 3S mq/1 Final: <br /> rngA <br /> Aeration: N/A Control Hardness Initial: 40 mg/I. Final: 4 S mg/I .Control Alkaline Initial: 30 mg/I .Final 35mg/I .Control Conductivity 2 I mhoskrn <br /> Aeration Rate: N/A Aeration Duration: Tanks: / <br /> Bioassay Date Control Dilution <br /> Conditions Time 750 500 250 DUP 750 DUP 500 DUP 250 <br /> No. % No-7-' % I No. % No. % No. °a No. % No. % <br /> 0 4 30 10 100 10 100 10 100 10 100 10 100 10 100 10 100 <br /> 0S/01 10 100 10 f00 (O f 0 10 100 1 10 (00 10 100 to too <br /> Organisms 0'r-, 0X 10 100 10 100 IO top 10 100 q 0 t0 (OO (O (00 <br /> Surviving os o3 10 100 9 D (O too to 100 10 100 10 (00 <br /> 0,V0 i. 10 too a 10 too 10 t00 0 10 too 10 too <br /> OG : 40 6.6 6. 6 .1. 6. 6.a D- 6.a, <br /> Dissolved ( 4- 106.9 1 _ 6.6 16.S 6.::r 616 .(O.s 6.4 <br /> Oxygen 1 S,o O 1.a 6--4 E. 6.9 6. ' 6. <br /> mg/L 1-3 30 ':1,-O 6.3 6- 6. -3 6 6 <br /> I t 'a-D 11. t 6 16.9 I.Q� 6 <br /> Start ! . 1 (9. °C q.6 19- °C : --E.� (9. °C (9. °C .6 t9-;f 0C 19.x-°C 19.E°C ; <br /> p1.1 24 Hr -4.4 a,o.3°C -:�_' O-3OC -4.3 jo.3°C 4.2 20.�,°C 't,4 PL 3 °C .3 °C ` "Z .910.3°C <br /> 48 Hr -4. 1 19.9°C `t_3 (9.9 °C 7T 113.,3°C 1. I (9.9 °C -4-3 11qA °C `.� 19.(3 °C -( (q,,3 °C <br /> Temp 72 Hr -4-.1)- �P.-)_°C `f.P- 2 .-D °C . ':T.0 0..2°C 1.0 -Zo. °C 4-D- R.O_Z°C -4�O O.a °C -4.o °C <br /> 96 Hr -4.,;1- lit. °C 1q,9°C . '4.I 19-S°C 4.1C)I 19. °C I Iq,g °C 4 I IQ_ °C --47y-] °C <br /> Results-LC3o= > Sa mg/I Method of Calculations <br /> A <br /> > sa mg/I 95%Confidence Limits <br /> %Survival NIA LC50 Method <br /> i <br /> Toxic Units T.U. NIA <br /> Observation/Remarks Laboratory Supervisor v <br />