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FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY Rquac <br /> Testing <br /> Laboratories <br /> Lab No.: A 1 7 t7 N� t v 3 - ora I <br /> Client/ID: T4 71 o - )g b t!y -A D- I <br /> TEST SUMMARY <br /> Species: Pimephales promelas. Source: In-Lab Culture. <br /> Fish weight(gm): av: 0,)7 ; min:0. )l ; max: 0•'l3 . Regulations: CCR Title 22. <br /> Reference Toxicant: SDS conducted monthly. Test Protocol: California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints: Survival at 96 hrs. <br /> Temperature: 20+/-2°C. Test type: Static. <br /> Aeration: none, unless D.O. drops below 5.0 mg/I. Feeding:None. <br /> Number of replicates: 2. Number offish per chamber: 10. <br /> Dilution water: Soft reconstituted water(40-48 mg/l CaCO3). Photoperiod: 16/8 hrs light/dark. <br /> TEST DATA <br /> INITIAL 24 Hr 48 Hr 72 Hr 96 Hr <br /> Date/Time: Y-(3-17 [o o -( -tz fV>VS-/ 7 WD -!r;-! to)& -(7-( ) IUj�J <br /> Analyst: <br /> °C I DO I pH °C I DO pH #D 11 °C DO pH #D °C DO pH #D °C I DO I pH #D <br /> Control A -& 0 C.,jl?.J g.3 $ D U $.3 o� $ 3 G u ,F'3 3'0 <br /> Control B g'+3 g,o `l 3 8• o c� I �.� l�• v 6 zato fS`t �'.v G <br /> 400 mg/l A y ! Q.o lo, $ l 7$ 0 Zr 7 q o. 8. f,D 0 Z0 s <br /> 400 mg/1 B1 �, p 20. g.r P-S c ( p•a 0 S -S <br /> 750 mg/1 AI S10 Q r q 0 126. l• 3 �-g 0 .2 e, t g 0 ZG+�B``�12G <br /> 750 mIB $�1 8.0 Q-r Q o. 7- R- 12-EC--d <br /> Comments: Extraction method: Mechanical shaking i/. <br /> None(aqueous solution) ^M. <br /> Dissolved Oxygen(DO)readings in mg/l 0, Test Aerated: � / No <br /> CONTROL HIGH CONCENTRATION Total Number Dead <br /> Alkalinity Hardness alkalinity Hardness Control /20 <br /> Initial 1( mg11 CaCO, s(f mg/1 CaCO, 3 r mg/1 CaCO, ( mg/!CaCO, 400 mg/1 0 /20 <br /> Final 3 I mg/1 CaCO, mg/1 CaCO, 3 mg/1 CaCO, 7 mg/1 CaCO, 750 mg/l /20 <br /> RESULTS <br /> the checked ✓ result applies based on fish survival rates of this test; NA- notapplicable) <br /> PASSED LC50>750 mg/1 (<40% dead in 750 mg/I conc.) <br /> /`/4- FAILED z40% dead in 750 mg/l (close to passing-definitive test recommended) <br /> FAILED LC50<400 mg/I (>60%dead in 400 mg/l conc.) <br /> Page 44 of 53 4/19/2017 <br />