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Table 3.1 Page 2 of 2 Data Characterization and Statistical Comparisons New Wood Rail Ties and Untreated Controls Union Pacific Railroad(UPRR) Untreated ALX Copper Creosote DEN Hope Lit Controls(n=5) Treated(n=10) Naphthanate(n=2) Treated(n=8) Treated(n=10) Treated(n=5) Treated(n=10) Analyte Criterion Unit Minimum Maximum Minimum Maximum #>Control Minimum Maximum #>Control Minimum Maximum #>Control Minimum Maximum #>Control Minimum Maximum #>Control Minimum Maximum #>Control Azobenzene mg/kg ND(1.34) ND(13.4) ND(26.8) ND(53.6) N/A ND(53.6) ND(53.6) N/A ND(53.6) ND(53.6) N/A ND(5.36) ND(53.6) N/A ND(26.8) ND(26.8) N/A ND(26.8) ND(107.2) N/A Benz(a)anthracene mg/kg ND(0.88) 13 110 1100 10 55J 62J 2 29J 360 8 86 3200 10 220 1400 5 460 2500 10 Benzidine mg/kg ND(0.46) ND(4.6) ND(9.2) ND(18.4) N/A ND(18.4) ND(18.4) N/A ND(18.4) ND(18.4) N/A ND(1.84) 25J 1 ND(9.2) ND(9.2) N/A ND(9.2) ND(36.8) N/A Benzo(a)pyrene mg/kg ND(1) 8.91 58J 440 10 79J 79J 2 73J 170J 8 36 1200 10 110 430 5 260 1200 10 Benzo(b)fluoranthene mg/kg ND(1.08) 10 69J 540 10 65J 67J 2 ND(21.6) 170J 7 44 1500 10 110 550 5 320 1200 10 Benzo(g,h,i)perylene mg/kg ND(0.58) ND(5.8) 191 120J 9 ND(23.2) ND(23.2) N/A ND(23.2) 110J 5 101 310 10 34J 100 5 94J 320 10 Benzo(k)fluoranthene - mg/kg ND(0.64) ND(6.4) 45J 430 10 39J 42J 2 ND(25.6) 120J 7 38 1400 10 901 520 5 200 930 10 Benzoic acid - mg/kg ND(0.72) ND(7.2) ND(14.4) ND(28.8) N/A ND(28.8) ND(28.8) N/A ND(28.8) ND(28.8) N/A ND(2.88) ND(28.8) N/A ND(14.4) ND(14.4) N/A ND(14.4) ND(57.6) N/A Benzyl alcohol - mg/kg ND(0.72) ND(7.2) ND(14.4) ND(28.8) N/A ND(28.8) ND(28.8) N/A ND(28.8) ND(28.8) N/A ND(2.88) 35J 2 ND(14.4) ND(14.4) N/A ND(14.4) ND(57.6) N/A Bis(2-chloroethoxy)methane - mg/kg ND(0.3) ND(3) ND(6) ND(12) N/A ND(12) ND(12) N/A ND(12) ND(12) N/A ND(1.2) ND(12) N/A ND(6) ND(6) N/A ND(6) ND(24) N/A Bis(2-chloroethyl)Ether - mg/kg ND(0.5) ND(5) ND(10) ND(20) N/A ND(20) ND(20) N/A ND(20) ND(20) N/A ND(2) ND(20) N/A ND(10) ND(10) N/A ND(10) ND(40) N/A Bis(2-chloroisopropyl)Ether - mg/kg ND(0.34) ND(3.4) ND(6.8) ND(13.6) N/A ND(13.6) ND(13.6) N/A ND(13.6) ND(13.6) N/A ND(1.36) ND(13.6) N/A ND(6.8) ND(6.8) N/A ND(6.8) ND(27.2) N/A Bis(2-ethylhexyl)phthalate - mg/kg ND(1.04) ND(10.4) ND(20.8) ND(41.6) N/A ND(41.6) ND(41.6) N/A ND(41.6) ND(41.6) N/A ND(4.16) 65J 7 ND(20.8) 33J 1 ND(20.8) ND(83.2) N/A Butylbenzyl Phthalate - mg/kg ND(0.88) ND(8.8) ND(17.6) ND(35.2) N/A ND(35.2) ND(35.2) N/A ND(35.2) ND(35.2) N/A ND(3.52) 78J 5 ND(17.6) ND(17.6) N/A ND(17.6) ND(70.4) N/A Carbazole - mg/kg 0.73J 12 92J 930 10 21J 23J 2 ND(18.4) 280 7 78 3200 10 230 1200 5 520 3000 10 Chrysene - mg/kg ND(0.8) 13 120 1000 10 51J 59J 2 27J 300 8 83 2800 10 220 1300 5 430 2200 10 Dibenz(a,h)anthracene - mg/kg ND(0.42) ND(4.2) ND(8.4) ND(16.8) N/A ND(16.8) ND(16.8) N/A ND(16.8) ND(16.8) N/A ND(1.68) ND(16.8) N/A ND(8.4) 58J 1 ND(8.4) 67J 1 Dibenzofuran - mg/kg 1.91 16 400 3500 10 1801 1901 2 87J 1200 8 180 5400 10 720 2800 5 960 4600 10 Diethyl phthalate - mg/kg ND(0.48) ND(4.8) ND(9.6) ND(19.2) N/A ND(19.2) ND(19.2) N/A ND(19.2) ND(19.2) N/A ND(1.92) ND(19.2) N/A ND(9.6) ND(9.6) N/A ND(9.6) ND(38.4) N/A Dimethyl phthalate - mg/kg ND(0.44) ND(4.4) ND(8.8) ND(17.6) N/A ND(17.6) ND(17.6) N/A ND(17.6) ND(17.6) N/A ND(1.76) ND(17.6) N/A ND(8.8) ND(8.8) N/A ND(8.8) ND(35.2) N/A Di-n-butyl phthalate - mg/kg ND(1.34) ND(13.4) ND(26.8) ND(53.6) N/A ND(53.6) ND(53.6) N/A ND(53.6) ND(53.6) N/A ND(5.36) ND(53.6) N/A ND(26.8) ND(26.8) N/A ND(26.8) ND(107.2) N/A Di-n-octyl phthalate - mg/kg ND(0.46) ND(4.6) ND(9.2) ND(18.4) N/A ND(18.4) ND(18.4) N/A ND(18.4) ND(18.4) N/A ND(1.84) 191 5 ND(9.2) ND(9.2) N/A ND(9.2) ND(36.8) N/A Fluoranthene - mg/kg 2.3J 46 630 5600 10 370 390 2 180 B(i)J 2200 8 310 9400 10 1200 5800 5 1700 8400 10 Fluorene - mg/kg ND(1.08) 21 450 3900 10 1901 200 2 87J 1300 8 220 6500 10 820 3700 5 1100 5300 10 Hexachlorobenzene 0.13 mg/kg ND(0.3) ND(3) ND(6) ND(12) N/A ND(12) ND(12) N/A ND(12) ND(12) N/A ND(1.2) ND(12) N/A ND(6) ND(6) N/A ND(6) ND(24) N/A Hexachlorobutadiene 0.5 mg/kg ND(0.78) ND(7.8) ND(15.6) ND(31.2) N/A ND(31.2) ND(31.2) N/A ND(31.2) ND(31.2) N/A ND(3.12) ND(31.2) N/A ND(15.6) ND(15.6) N/A ND(15.6) ND(62.4) N/A Hexachlorocyclopentadiene -- mg/kg ND(0.28) ND(2.8) ND(5.6) ND(11.2) N/A ND(11.2) ND(11.2) N/A ND(11.2) ND(11.2) N/A ND(1.12) ND(11.2) N/A ND(5.6) ND(5.6) N/A ND(5.6) ND(22.4) N/A Hexachloroethane 3.0 mg/kg ND(0.86) ND(8.6) ND(17.2) ND(34.4) N/A ND(34.4) ND(34.4) N/A ND(34.4) ND(34.4) N/A ND(3.44) ND(34.4) N/A ND(17.2) ND(17.2) N/A ND(17.2) ND(68.8) N/A Indeno(1,2,3-cd)pyrene -- mg/kg ND(1.8) ND(18) ND(36) 170J 9 ND(72) ND(72) N/A ND(72) 120J 5 16J 510 9 53J 150 5 130 490 10 Isophorone - mg/kg ND(0.5) ND(5) ND(10) ND(20) N/A ND(20) ND(20) N/A ND(20) ND(20) N/A ND(2) ND(20) N/A ND(10) ND(10) N/A ND(10) ND(40) N/A Naphthalene - mg/kg 2.5J ND(5) 490 5400 10 66J 70J 2 ND(20) 510 7 84 4600 10 1800 3800 5 520 3200 10 Nitrobenzene 2.0 mg/kg ND(0.42) ND(4.2) ND(8.4) ND(16.8) N/A ND(16.8) ND(16.8) N/A ND(16.8) ND(16.8) N/A ND(1.68) ND(16.8) N/A ND(8.4) ND(8.4) N/A ND(8.4) ND(33.6) N/A N-Nitrosodimethylamine(NDMA) -- mg/kg ND(0.68) ND(6.8) ND(13.6) ND(27.2) N/A ND(27.2) ND(27.2) N/A ND(27.2) ND(27.2) N/A ND(2.72) ND(27.2) N/A ND(13.6) ND(13.6) N/A ND(13.6) ND(54.4) N/A N-Nitrosodi-n-propylamine(NDPA) - mg/kg ND(0.52) ND(5.2) ND(10.4) ND(20.8) N/A ND(20.8) ND(20.8) N/A ND(20.8) ND(20.8) N/A ND(2.08) ND(20.8) N/A ND(10.4) ND(10.4) N/A ND(10.4) ND(41.6) N/A N-Nitrosodiphenylamine - mg/kg ND(0.48) ND(4.8) ND(9.6) ND(19.2) N/A ND(19.2) ND(19.2) N/A ND(19.2) ND(19.2) N/A ND(1.92) 22J 4 ND(9.6) ND(9.6) N/A ND(9.6) ND(38.4) N/A Pentachlorophenol 100 mg/kg ND(1.1) ND(11) ND(22) ND(44) N/A ND(44) ND(44) N/A ND(44) ND(44) N/A ND(4.4) ND(44) N/A ND(22) ND(22) N/A ND(22) ND(88) N/A Phenanthrene -- mg/kg 3.2J 65 880 7700 10 530 550 2 270 3200 8 480 15000 10 1900 8600 5 2600 15000 10 Phenol - mg/kg ND(0.52) ND(5.2) ND(10.4) 35J 5 ND(20.8) ND(20.8) N/A ND(20.8) ND(20.8) N/A ND(2.08) 29J 2 47J 110 5 ND(10.4) ND(41.6) 1 Pyrene - mg/kg 1.7J 31 410 3500 10 240 250 2 120 B(i)J 1400 8 210 6300 10 810 3700 5 1100 5500 10 Pyridine 5 mg/kg ND(0.42) ND(4.2) ND(8.4) ND(16.8) N/A ND(16.8) ND(16.8) N/A ND(16.8) ND(16.8) N/A ND(1.68) ND(16.8) N/A ND(8.4) ND(8.4) N/A ND(8.4) ND(33.6) N/A Total Cresol 200 mg/kg ND(8) ND(80) ND(160) ND(320) N/A ND(320) ND(320) N/A ND(320) ND(320) N/A ND(32) ND(320) N/A ND(160) ND(160) N/A ND(160) ND(640) N/A B)FISH BIOASSAY LC50 500 mg/L >750 >750 >750 >750 N/A >750 >750 N/A >750 >750 N/A Hazardous >750 2 >750 >750 N/A Hazardous >750 3 LC50 Definitive 500 mg/L N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 29.5 >650 2 N/A N/A N/A N/A N/A N/A Number Failing Bioassay(<500) n/a n/a 0 0 0 0 2 0 3 Note: Highlighted cells indicate analytes present at concentations elevated above controls in treated wood samples. (In one or more treatment type groups,typically in half or more of the samples analyzed). GHD 11183843Pollack-1-T3.1-3.2.xlsx