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R <br />Biological indicator testing is done onep er week with the first <br />load of biological waste of the day. <br />2. Place culture in a small box, tape box so that culture does <br />not fall out. <br />3. Place the box in an autoclave bag with red bag trash and tie <br />off the bag. <br />4. Pressure gauge on sterilizer must be checked and recorded <br />before proceeding with the process. Pressure gauge reading <br />should be above 72psi <br />5. At the completion of sterilization cycle, biological indicator <br />test indicator is taken to Central Supply for incubation and <br />result recorded in a 3 TM log book as prescribed in <br />California Health and Safety Code: Section 118215 (E). <br />6. Incubation time for control test is 3 to 5 hours before reading <br />and documented. <br />7. Control has to be kept for 24 hours after the test is <br />completed. <br />A heat sensitive tape is inserted in autoclave unit to record each sterilization process <br />to indicate the attainment of required sterilization conditions as described in <br />California Health and Safety Code: Section 118215 (D). In the event that a load is <br />deemed inadequately sterilized, the above procedure is repeated until satisfactory <br />result is attained to meet California Health and Safety code. If failed of sterilization is <br />due to equipment failure, an outside vendor specialized in particular equipment shall <br />be called in. The vendor is expected on the facility premises within 2 hours from the <br />first call initiated. <br />B. Listing of specific materials needed for procedure. <br />1. Control sample (unsterilized indicator) <br />2. Actual biological indicator (placed in sterilizer) <br />3. 3M Attest T Rapid Biological indicator log book <br />4. 3M Attest TM Rapid Biological indicator incubator <br />VII. References <br />A. Authored by Gloria Alvarez <br />B. Revised by Hawa Suah,MS-HCA, MPA <br />National Standards and Recommended Practices for Sterilization. Association for the <br />Advancement of Medical Instrumentation, Arlington, Virginia, Vol 1.1-1.2, 1995. <br />CDC Control and prevention, Sterilization — Monitoring, March 4, 2010 <br />