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Infectious material continued -3- <br />L. Restrict Al in and out of the TB room® ® <br />testingM. Health of personnel - TB lab. personnel should be healthy. Fatigue, <br />emotional stressf malnutrition all reduce resistance to TB. Tuberculin <br />on negative reactors# imonths.X-rays <br />clothing.should be taken on positive reactors every ye�r. <br />N. Accidental contamination - cover area with phenol (ophene) soaked paper <br />towels. Flood with phenol (ophene). Leave hood fan running and evacuate <br />personnel from the room for 30 minutes. <br />IX. Precautions: hepatitis antigen and antibody testing <br />A. Gloves should be worn� <br />B. Cover street clothes - disposable gowns are recommended to cover all <br />C. Cover work areas with plastic -backed absorbent paper. Dispose of this <br />by autoclaving when finished. <br />D. Clean ► all spills♦ # r to stand 1 <br />E. Autoclave all disposable material usei before <br />F. Autoclave all reagent bottles containing antigen before discarding. <br />X. All specimens collected from possible hepatitis patients should be marked <br />with yellow preprinted tape. <br />;qM 1101 <br />A. Contaminated culture specimensand media. <br />1• Place all,„material for disposal in autoclavable bags in containers <br />marked TO BE AUTOCLAVED. <br />Specimen2. ; centrifuge supernatants will be discarded into the dis� <br />posable containers provided. Pour some phenol into container before <br />3. •. housekeeper will pick up cont <br />material # autoclave.N: All material fromthe Mcobacterium <br />lab._including ss paper basketbe s to the autoclave by <br />the tech who is alre - ady masked and_L2.wned, at the end of working day. It <br />is to be placed in autoclave and sign "Not Autoclaved TB Material” placed <br />in # <br />4. All material, with the following exception , will be autoclaved 15 <br />minutes with 15 lb. pressure, at 121 degrees C. Mycobacteriology lab. <br />material will be autoclaved for 45 minutes. Indicator tags will be <br />placed on all bags to insure that material is decontaminated., All <br />material that is disposable is to be placed in a paper Ecolo bag. When <br />full the bags will'be stapled shut and removed to the trash compactor. <br />The trash compacted material is removed to a sanitary landfill disposal <br />site ♦ dumped. <br />5. All fungus cultures are R; in a metal can marked "FUNGUS CULTURE <br />TO BE AUTOCIAVED". are autoclaved as above on three different <br />days to insure decontamination. If disposable discard as above <br />B. Blood specimens for discard and all other blood contaminated material: <br />1. This will be placed in waxed cartons in the lab. department marked <br />"TO BE 1 <br />