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1534 Willow Pass Road,Pittsburg,CA 94565-1701 <br /> M c C a m p b e I l Analytical, Inc. Toll Free Telephone:(877)252-9262/Fax:(925)252-9269 <br /> "When Quality Counts" http://www.mccampbell.com/E-mail:main@mccampbell.com <br /> Analytical Report <br /> Client: UniFirst Corporation WorkOrder: 2509398 <br /> Date Collected: 09/05/2025 7:30 Extraction Method: Polisini&Miller(CDFG 1988)(Haz.) <br /> Date Received: 09/05/2025 16:25 Analytical Method: Polisini&Miller(CDFG 1988)(Haz.) <br /> Project: Samples received 9/5/25 Sample Matrix: Solid <br /> Hazardous Waste 96-hr Definitive Bioassay w/Fathead Minnows <br /> Lab ID 2509398-002A Species Pimephales promelas Avg.Length(mm) 30.454 <br /> Client ID DAF Sludge Common Name Fathead Minnow Avg.Weight(g) 0.281512 <br /> Control Water Batch# EPASW 550 Organism Supplier ABS Max Weight(g) 0.34861 <br /> Control Water Soft Synthetic Water Organism Lo # 250912FHM Min Weight 0.23923 <br /> Randomization Ne N/A Test Location TR 2.2A <br /> Initial Final <br /> Control 750 mg/L Control 750 mg/L <br /> Hardness(mg/L as CaCO3) 44 48 48 48 <br /> Alkalinity(mg/L as CaCO3) 34 37 37 38 <br /> Fish1D 1 2 3 4 5 6 7 8 9 10 <br /> Length(mm) 33.04 30.15 30.08 30.39 28.14 30.12 29.78 31.29 32.97 28.58 <br /> Weight(g) 0.34861 0.28262 1 0.23923 0.31905 0.25176 0.26175 0.26133 0.28281 0.31115 0.25681 <br /> Hazardous Waste Bioassay Sample Preparation Log <br /> Sample Prep Date I Balance ID Sign-Off Date/Time On Shaker Sign-Off Date/Time On Shaker I Sign-Off <br /> 9/16/2025 1 BL 38 JAD 9/16/2025 5:51:00 PM I MTO 9/17/2025 9:09:00 AM I MTO <br /> Conclusion: 'NON-HAZARDOUS'The LCeo of this sample is greater than 500 mg/L.Therefore the sample should not be considered <br /> hazardous on the basis of aquatic toxicity per the guidance established in the Polisini&Miller(CDFG 1988)protocol.Per guidance <br /> provided in CCR,Title 22,Section 66261.24a.2B.6,samples with an acute 96-hour LCso value greater than 500 mg/L when measured <br /> in soft water with fathead minnows are not identified as toxic hazardous waste to the aquatic environment. <br /> 96 LC50: >750 mg/L LC50 Method: Linear Interpolation(ICPM <br /> 95%Lower Confident Limit: N/A 95%Upper Confident Limit: N/A <br /> CA ELAP 1644•ORELAP(NELAP)4033 <br /> Page 11 of 19 <br />