ARCHIVED REPORTS XR0000090 - Laserfiche WebLink

Home Browse Search

' - 2 Percentage of Strain T es in Each Sam le: Sample=- MW-9 MW-12 MW-13 Description Strain 1 60% _ 40% Med, tan, shiny ' 2 1 °k 30% Med, white, shiny 40% _ � large,pale yellow 4 _ _ 30% White,mucosal 1 GC-FAME & BiologTm Processing: Following isolation the strains were individually streaked onto TSA. The TSA plates were ' processed after 24 hour incubation by[Method 1 - Standard GC-FAME]. The strains were examined against both the Aerobe (TSBA [rev. 3.80]) and Clinical Aerobe (CLJN frev.3.80])GC- FAME databases. Subsequently the strains were prepared for Bioloem analysis by suspending ' them in sterile saline and loading the solutions into the appropriate microtiter plates (Gram negative or Gram positive). The plates were incubated for 24 hours and then examined against version 3.5 of the Bioloe database using an automated microplate reader. ' Summary of GC-FAME/Biolog Analyses MStrain Primary Sim. Dist Primary ID by Plate Sim. Dist. Name IdentifGCion by Coef. Coef. Biology Type Coef Coef ' 2600-1 Pseud°monas 0.625 3.135 Pseudomonas 0 973 0.288 chlororaphis fluorescens type A GN 2600-2 Acmetobacterjohnsonu 0.194 585 Acinetobacter GN 0 674 3 946 ohnsomi/genospecies 7 ' 2600-3 Pseudomonas syringae 0.355 4 654 Pseudomonas GN 088 1679 �gae fluorescens type A ' 2600,4 Pseudomonas 0 238 5 473 Pseudomonas GN 0 632 4 746 savastanoi pv frazinus fluorescens type G Similarity and Distance Coefficient In order to create the database that we use to identify your organisms, thousands of species of bacteria had to be tested. In fact each species itself had to be tested hundreds of tunes to determine a set of characteristics unique to it. The species characteristics that are in our database are an "average"of the characteristics of hundreds of tested bacteria of the same species The Similanty and Distance Coefficient of your organism refers to the similarity and distance to the hypothetical `mean' organism m the database.The database organism has a similarity coefficient of one and a distance of zero So the closer your strain is to one and zero the more closely it matches the mean organism in the database. A good match is one with a similarity coefficient greater than .5 and a distance coefficient of less than 7.