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xS yA p t}7] r aK `'Za,`•3� ,v2„' #� -� \J'�'4F• "T h v^�i+ ,inv .TL..vlC'_ c ,.9i v� known turbidirnetric standard The bacterial suspension is inoculated into the microplate wells (150 pl per well) and the plate covered with the microplate hd The covered plates are the MiL, inc. incubated at 28-35°C for 4 hours or overnight Interpretation of the Carbon Source (16-24 hours) Should other diluents be re- Pattern Recognition Data using a quested or used, such changes will be noted Multi-well Plate Method (Biolog Microplates may be read at 4 or 24 hours be- Microplate SystemTM ) -- Contact Us: cause some organisms give results at 4 hours and 314-878-6626 or Fax 314-878-9376 may become unreadable at 24 hours The plates are read in our microplate reader at 590 nm The ' The MiL, inc utilizes the Biolog Microplate absorbance or transmittance (i.e color) in each SystemTMfor microbial identification and charac- well is referenced against the negative control terization by carbon source pattern recognition well (A-1)so that any purple color recorded The microplate technique allows for character- above this control level is read as a positive ization by 95 different tests yielding a potential utilization of the given carbon source. The data of 4 x 1028 patterns generated from a single are reported as the percent color change as ' microplate. Each strait of micro-organum compared to well A-1 utilizing the following yields a distinct pattem and the different species formula. of bactena will give distinct families of patterns that can be recognized by the Biolog MLcroLoem Software. Microplates are avail- Percmt color change=OU59Nwrl11-QW (well n-in able for Gram Negative (GN), Gram Positive ODSMwen n-1) (GP) and E.coli/Salmonella (ES) Analysis. Custom analysis are performed by the MiL, inc. positive results will be reported in brackets (I ]), and can be particularly useful in biodegradation generally if the Percent Color Change is equal to or additional selective media development or greater than 40, the reaction in the given well studies. Additional interpretative instructions is considered to be "positive" however the ' are provided with such custom services. parameters for each substrate may be different and a positive test below a value of 40 is pos- To characterize a given microbial isolate the sible. The reported results will be otherwise ' - organism is streaked onto a nutrient medium that considered negative. The computer algorithms will support vigorous growth (for example, employed provide standardization of settings Nutrient agar, tryptic soy agar or tryptic soy agar ensuring repeatability and avoidance of operator ' supplemented with 5% sheep red blood cells), bias. Names of all carbon source substrates The more fastidious organisms may require employed are provided in the results regardless chocolate or BHI agar for growth, whereas many of response. ' environmental organisms grow better in more minimal media. The culture plates are incubated We, the MLL's microbiologists, find these meth- at 28 to 35° C for 418 hours (environmental ods to be excellent for strain characterization or isolates are typically grown at 28°C with differentiation between isolates However, we thermophylhc strains often incubated at 50°Q. urge caution in acceptance of the putative identi- After incubation colonies are removed from the ficattons to the commercial database and suggest culture plate using a saline moistened cotton these tests be conducted in conjunction with . swab A suspension of uniform turbidity is other methods (we recommend our GC-FAME prepared in 0.85% saline by comparison with a analyses) when strain identifications are sought