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03/25/2004 14:15 4737293 PETAL PAGE 04 <br /> - •�� •, ��rt twv i,120NnENTwI 92qe6e399 P.92 <br /> r i L INTRODUCTION ! <br /> • : <br /> . !:: The Callfomia Department of substances Control has adopted regulations(R-4 -78) which <br /> x"k` define criteria for the identification of hazardous wastes. These criteria are codi led in Chapter <br /> 11,Miole 3 of Title 22 of the California Code of Regulations, Toxicity to aq ie life <br /> s'pecifteaily fish, is one of the criteria used to gauge the hazardous potential of a ate. An acute <br /> ,^ 96-hour bioassay is used to determine the LC50 as defined in Section 66261.24 )(6) of these <br /> regulptions. This 96-hour LC50 value serves as the numerical indicator of the t xicity of was <br /> N.. <br /> to ag0tic life. The sainple is deemed hazardous if the LC50 is less than 500 m /L. <br /> 's This 6;: <br /> report describes the eP procedures usedand the results obtained for the h us waste <br /> _< aquatic toxicity screening test(s)performed by Block Environmental Services ES)for <br /> Precision Enviro-Tech, <br /> AES is an Environmental Laboratory Accreditation Program certified laborato <br /> } ! 2. MATERIALS AND METHODS <br /> 2.1 TEST 01WAN19 YLS <br /> Rainbow trout(Oncerhynchus mykiss),obtained from a commercia supplier. <br /> ..... <br /> 2.2 TEST PROCEDURES <br /> ...: <br /> Adetailed <br /> procedure for this test is outlined in laboratory standard operating pri ccdutt s(SOPS <br /> kept ht the BES laboritory. These SOPS ate based upon the following referen s: <br /> • California's Title 22 Code,Section#66261,24(x)(6); Static Acute 8 oassay <br /> e ;i Proceduresfor HazardousWaste Samples, Poli" and Miller, 198 California <br /> k Department of Fish and Came <br /> `t i • <br /> Guidelinesfor Perforating Static Acute Toxicity Fish Bioassays in unicipal and <br /> i; . . bdustrial Wastewaters,Kopperdahl, 1976,California Department o Fish and Ganl <br /> i. • Standard Methods for the Examination of Water and Wastewater, 1 m Edition, <br /> l ',r, t• American Public Health Association, 1995. <br /> ^ J IATA ANALYSIS <br /> . All tpxioity testing results will be analyzed using an appropriate statistival metj od to detertni4 a <br /> i 5 <br /> LC56 drid the corresponding 95%confidence limits, <br /> ' HES 921120 <br /> j Page 2 of 3 <br /> I <br />