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re <br /> 1. INTRODUCTION <br /> 1 <br /> '1 The California Department of Toxic Substances Control has.adopted regulations (R-45-78) which <br /> define criteria for the identification of hazardous wastes, These criteria are codified in Chapter 11, <br /> x Article 3 of.Title 22 of the California Code of Regulations. Toxicity to aquatic life, specifically <br /> fish, is one of the criteria used to gauge the hazardous potential of a waste. An acute 96-hour <br /> bioassay is used to determine the LC50 as defined in Section 66261.24(a)(6) of these regulations. <br /> This 96-hour LC50 value serves as the numerical indicator of the toxicity of a waste to aquatic life. { <br /> The sample is deemed hazardous if the LC50 is less than 500 mg/L. <br /> This.report describes the procedures used and the results obtained for the hazardous waste aquatic <br /> toxicity screening test(s)performed by Block Environmental Services (BES) for,TestAanerica San <br /> Francisco. <br /> I <br /> SES is an Environmental Laboratory Accreditation Program certified laboratory(#1812). <br /> 2. MATERIALS AND METHODS <br /> 2.1 TEST ORGANISMS <br /> • Rainbow Trout, Oncorhynchus mykiss, obtained from a commercial supplier. <br /> P <br /> 2.2 TEST PROCEDURES <br /> ` A detailed procedure for this test is outlined in DPHS-ELAP approved standard operating <br /> procedures(SOPs), which are on file at the BES laboratory. These SOPS are based upon the <br /> 4 <br /> following references: <br /> • California's Title 22 Code, Section#66261.24(a)(6);Static Acute Bioassay Procedures <br /> for Hazardous Waste Samples, Polisini and Miller, 1.988, California Department of Fish <br /> and Game <br /> f + Guidelines for Performing Static Acute Toxicity Fish Bioassays in Municipal and <br /> Industrial Wastewaters,Kopperdahl, 1976, California Department of Fish and Game <br /> • Standard Methods for the Examination of Water and Wastewater, 19"'Edition,American <br /> Public Health Association, 1995. <br /> E. <br /> BES #27263 Page 2 of 3 <br /> Page 25 of 30 <br />