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Sequoia 680 Chesapeake Drive Redwood City,CA 94063 (650) 364-9600 FAX(650)364-9233 <br /> 404 N.Wiget Lane Walnut Creek,CA 94598 (510) 988-9600 FAX(510)999-9673 <br /> Analytical 819 Striker Avenue,Suite 8 Sacramento,CA 95834 (916) 921-9600 FAX(916)921-0100 <br /> iirrjo <br /> .......... .......... <br /> ........ . ...... .... <br /> Pacific Environmental Group Client Project ID: Unocal #1205, Lathrop, CA Sampled: Nov 18, 1997 <br /> 11315 Sunrise Gold Circle, Ste. M Sample Descript: Water,Tank Received: Nov 19, 1997 <br /> Rancho Cordova, CA 95742 Analysis Method: See below <br /> Attention: Rusty Benkosky Lab Number: 711-1173 Reported: Dec 1, 1997 <br /> STATIC HAZARDOUS ABBREVIATED SCREEN BIOASSAY <br /> Species: Pimephales promelas W. <br /> Common Name: Fathead minnow fall Organisms/Tank: 10 <br /> Organisms/Conc.: 20 <br /> Mean length: 40 MM Min. length: 36 MM WEIVED Tank Depth: 13 cm <br /> Max. length: 43 MM Tank Volume: --To-L <br /> Min. weight: 0.28 g Acclimation Temp.: 20 OC <br /> Mean weight: 0.42 g Max. weight: o.5o g Supplier: Sticklebacks Unlimited <br /> Dilution Water: Synthetic Softwater <br /> Hardness 40-48 Alkalinity,mg/L I Hardness,mg-/L <br /> Initial Final Initial Final-I <br /> Control 34io 31 2 48 <br /> T _ <br /> 750 ppm 60 <br /> 1 <br /> 80 40 1 240 <br /> L 0 <br /> Duplicate 750 ppm 60 80 1401 220 <br /> Initial 24 Hr 48 Hr 72 Hr 96 Hr <br /> DATE 1 11/21/971 11/22/97 1 11/23/97 1 11/24/97 1 1�1/25/97 <br /> DO C pH DO C pH # M DO C pH # M DO C pH # MI DO I C pH # M Total <br /> mg/L Tem Units mg/L Tem Units Dead mg/ Tem Units Dead mg/L Tem r Units Dead i mg/4 Tem Units Dead Dead <br /> Control 8.8 19 7.4 7.0 1 J97.0 0 6.0 21 6.9 0 5.2 19 6.6 0 3.7 20 6.6 0 <br /> 750 ppm 8.9 19 7.6 7.2 19 7.0 0 6.2 21 6.8 0 5.2 19 6.6 0 2.8 20 6.5 0 <br /> 300 ppm 8.9 19 7.5 6.8 19 6.9 0 5.6 21 6.8 0 4.7 19 6.6 0 2.6 20 6.4 0 <br /> Duplicate, <br /> 750 ppm 1 8.81 191 7.61 7.31 191 7-01 0 1 6.01 21 1 6.81 0 1 5.1 1 19 6.61 0 1 3.11 20 1 6.5 0 <br /> 300 ppm1 8.91 191 7.51 7.31 191 6.91 0 1 5.91 21 1 6.81 0 1 4.71 19 1 6.61 0 1 2.91 20 1 6.9qo 0 <br /> 0 <br /> Pass x Fail <br /> Remarks The screen fails if > 40% of the fish die in the 750 ppm concentration. <br /> Analyst: C.Throm Method Reference: Static Acute Bioassay Procedures for Hazardous Waste Samples, <br /> November 1988, California Department of Fish and Game WPCL. <br /> SEQU IA ANALYTIC <br /> dU '�L, #1210 <br /> Q <br /> ina C Schneider <br /> Project <br /> Id ojectct Manager/Sacramento Laboratory 711-1173.PES <1> <br />