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STUDY NO. : 238-93 ASSAY START- 11/01/93 <br /> SPECIES: Pime hales promelas EXPOSURE: �./ <br /> 96 hours <br /> SAMPLE: SDS Ref Tox Test SOFT Batch 090893 Lab <br /> The binomial test shows that 12 . 5 and 50 can be used <br /> as statistically sound, conservative 95 percent confidence limits <br /> because the actual confidence level associated with these limits <br /> is greater than 95 percent. <br /> 25 can be used as an approximate LC50 for this set of data. <br /> -----------RESULTS CALCULATED USING THE MOVING AVERAGE METHOD---------- <br /> Span G LC50 95 Percent Confidence Limits <br /> 3 6. 572934E-02 22 .87834 18 .74524 28 . 95456 <br /> 2 . 1198137 23 . 54723 18 . 69278 29. 18284 <br /> 1 . 3484681 25 13 . 87341 31 . 09513 <br /> 1 .7002976 25 18 . 64277 169 . 3438 <br /> An LC50 calculated using the Moving Average method may not be a very good <br /> estimate if the span is much less than the number of concentrations . <br /> --------------RESULTS CALCULATED USING THE PROBIT METHOD--------------- <br /> Iterations G H Chi-Square Probability <br /> 5 . 1324919 1 . 7273731 . 8667473 <br /> Slope = 4. 477848 <br /> 95 Percent Confidence Limits = 2 . 847937 and 6 . 10776 <br /> 64'e0 = 23 . 23474 <br /> 95 Percent Confidence Limits = 18 . 88599 and 28 . 80504 <br /> ---------RESULTS CALCULATED USING THE TRIMMED SPEARMAN-KARBER METHOD----=-- <br /> Dose Percent Monotonic Trimmed <br /> :onc. =Ln (Conc) Dead Rel. Freq. Rel . Freq. <br /> 50 3 . 912023 95 . 95 1 . 0625 <br /> 25 3 . 218876 50 . 5 . 5 <br /> 12 . 5 2 .525729 15 . 15 6 . 250001E-02 <br /> 6 . 25 1. 832581 0 0 -. 125 * <br /> 3 . 1 1. 131402 0 0 -. 125 * <br /> Alpha = 10 % <br /> 3roups trimmed and therefore not used in estimating LC50 <br /> are marked with an asterisk above. <br /> LC50 = 23 . 82512 <br /> Estimated 95 Percent Confidence Limits <br /> Lower: 19 .06122 Upper: 29. 77965 <br /> Variance estimate = 1. 244172E-02 <br /> Joh <br />