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Sequoia 680 Ches1-01apeake Drive Redwood City,CA 94063 (415) 364-9600 FAX(415)364-9233 <br /> 404 N.Wiget lane Walnut Creek,CA 94598 (510) 988-9600 FAX(510)988-9673 <br /> Analytical 819 Striker Avenue,Suite 8 Sacramento,CA 95834 (916) 921-9600 FAX(916)921-0100 <br /> Star Building Systems Client Project ID: Waste Testing Sampled. Apr 28, 1997 <br /> 12101 E. Brandt Rd. Sample Descript: Solid, Mastic Received: May 1, 1997:' <br /> Lockeford, CA 95237 Analysis Method: See below <br /> Attention: Jeremy Ball Lab Number 705-0016 Reported. May 16, 1997 <br /> STATIC ACUTE HAZARDOUS WASTE BIOASSAY - DEFINITIVE <br /> Species: Pimephales promelas Organisms/Tank: 10 <br /> Common Name: Fathead Minnow Organisms/Conc.: 20 <br /> Tank Depth: 13 cm <br /> Mean length: 38 mm Mlll. length: 35 mm Tank Volume: 10 L <br /> Max. length: 41 mm Supplier: Sticklebacks Unlimited <br /> Mean weight: 0.72 g Min.weight: o.51 g Acclimation Temp.: 20 °C <br /> Max. weight: o.89 g <br /> Control Water: Synthetic Softwater Alkalinity, mg/L Hardness, mg/L <br /> Hardness 40-48 Initial Final Initial Final <br /> Control 25 29 40 39 <br /> 1000 ppm 28 31 42 46 <br /> Duplicate 1000 ppm 28 32 43 46 <br /> Initial 24 Hr 48 Hr 72 Hr 96 Hr <br /> DATE 05/08/97 05/09/97 1 05/10/97 05/11/97 05/12/97 <br /> DO C pH DO C pH # M DO C pH # M DO C pH # M DO C pH # M Total <br /> mg/l. Temr Units mg/ Temr Units Dead mg/ Ten-F Units Dead mg/ Temr Units Dead mg/L Temr Units Dead Dead <br /> Control 9.6 18 7.2 6.41 18 7.3 0 1 5.91 18 7.1 0 6.1 18 7.3 0 5.7 18 6.7 0 0 <br /> 1000 pprin 9.6 19 7.7 5.8 20L72 <br /> 0 5.0 19 7.2 0 4.4 19 7.3 0 4.2 19 6.9 0 0 <br /> 560 ppm 9.4 19 7.8 6.5 19 0 5.8 19 7.1 0 5.8 19 7.3 0 5.2 19 7.0 1 1 <br /> 320 ppm 9.4 19 7.8 6.5 19 0 5.6 19 7.1 0 5.1 19 7.2 1 4.9 19 7.0 1 2 <br /> 180 ppm 9.4 19 7.8 6.5 19 0 5.8 19 7.1 0 5.5 19 7.2 0 5.2 19 7.0 0 0 <br /> 100 ppm 9.4 19 7.8 6.3 19 0 5.7 19 7.1 0 5.5 191 7.21 0 1 5.21 191 7.01 0 0 <br /> Duplicate DO C pH DO C pH #M DO C pH #M DO C pH #M DO C pH #M Total <br /> mg/L Temr Unit mg/ Temr Units Dead mg/ Tem Units Dead mg/ Tem Units Dead mg/ Tem Units Dead Dead <br /> 1000 pp 9.4 19 7.7 5.8 19 7.2 0 5.2 18 7.1 0 5.0 19 7.4 1 4.3 19 6.9 0 do 560 ppm 9.4 19 7.8 6.2 19 7.2 0 5.4 19 7.1 0 4.8 19 7.3 1 4.5 19 7.0 0 <br /> 320 ppm 9.4 19 7.8 6.6 19 7.2 0 5.7 19 7.1 0 5.7 19 7.2 0 5.5 19 7.1 0 <br /> 180 ppm 9.5 19 7.8 6.5 19 7.2 1 6.2 19 7.1 0 5.7 19 7.2 0 5.0 19 7.0 0 <br /> 100 ppm 9.5 1917.8 6.0 19 7.2 0 6.4 19 7.1 0 5.3 19 7.2 t7 5.0 1- <br /> LC-50: >1000 ppm LC-50 Calculation Method: Moving Average <br /> LC-50 Dup: -TODDppm <br /> Remarks: <br /> Analyst: K. Anderson/ Method Reference: Static Acute Bioassay Procedures for Hazardous Waste Samples, <br /> D. Newcomb November 1988, California Department of Fish and Game WPCL. <br /> SEQUOIA ANALYTICAL, #1271 <br /> nda C. Schneider <br /> Project Manager/Sacramento Laboratory 705-01 <2> <br />