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2. MATERIALS AND METHODS <br /> 2.1 TEST ORGANISMS <br /> Bioassay was performed using: <br /> • Fathead Minnows,Pimephales,proinelas, obtained from Thomas Fish <br /> Company(Anderson, CA) <br /> 2.2 TEST PROCEDURES <br /> A detailed procedure for this test is outlined in laboratory standard operating procedures <br /> (SOPS)kept at the BBS laboratory. These SOPS are based upon the following references,. <br /> • California's Title 22 Code, Section##66261.24(a)(6); "Static Acute Bioassay <br /> Proceduresfor Hazardous Waste Samples", Polisini and Miller, 1988, <br /> California Department of Fish and Game <br /> • "Guidelinesfor Pcrforming Static Acute Tbxichty Tish Bioassays in Municipal <br /> and-Industrial Warte Waters", Kopperdahl, F.R„ 1976, California Department <br /> of Fish and Game <br /> • ".Standard Methods for the Etiamination of Rater and Wastmvater", 1 ath <br /> Edition, American Public Health Association, 1992. <br /> 2.3 DATA ANAL'�'SI5 <br /> All toxicity testing results will be analyzed using the Trimmed-Spearman Karher statistical <br /> method. This program determines a LC50 and the corresponding 95% confidence limits. <br /> 6£/LI 'd 99400£6019 'ON Sd.d BIRO 1190 N3d i4�9i 3BI 96-OL-O34 <br />