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BioVir <br /> ueoru�ow[s <br /> DATE: March 24, 2004 <br /> TO: File <br /> FROM: Rick Danielson, Ph.D. <br /> Laboratory Director /Vice President <br /> SUBJECT: B040485 A - E. <br /> Notes to samples B040485A through B040485E processing: <br /> 1. 0.1 mg sample was added to 1 L particle free water for initial suspension. <br /> 2. Dilutions were prepared of the 0.1 g / L suspensions based upon the observed <br /> turbidity of the suspended sample. <br /> B040455A 1:1000 <br /> B040485B 1:1000 <br /> B040485C 1:100 <br /> B040485D 1:100 <br /> B040485E 1:1000 <br /> 3. It was observed that the samples contained magnetic particles. The particle counting <br /> instrument has a magnetic base. Although care was taken to minimize contact time to <br /> about 1 minute, there may have been magnetically-induced migration to the bottom of <br /> the assay vessel. <br />