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Pacific EcoRisk Environmental Consulting & Testing <br /> 1.INTRODUCTION <br /> Under contract to GeoAnalytical Laboratories, Inc. (GeoAnalytical),Pacific EcoRisk(PER) <br /> conducted a screening level Title 22 "Hazardous Waste" fish toxicity evaluation of a solid waste <br /> sample designated"Trailer Trash".This toxicity test consisted of a 96-hr static bioassay with <br /> juvenile fathead minnows (Pimephales promelas).This report describes the performance and <br /> results of this test. <br /> 2.ACUTE TOXICITY TEST PROCEDURES <br /> The methods used in conducting this test followed the guidelines established by the California <br /> Dept. of Fish & Game manual "Static Acute Bioassay Procedures for Hazardous Waste Samples" <br /> (Polisini and Miller 1988), with additional guidelines as provided by the California Dept. of <br /> Health Services (Janna White,personal communication). <br /> 2.1 Receipt and Handling of the Waste Sample <br /> A solid waste sample designated "Trailer Trash" was delivered to the PER testing laboratory on <br /> November 19,2013,where it was placed under refrigeration at 4°C until being used to prepare <br /> the test solutions.The chain-of-custody record for the collection and delivery of this waste <br /> sample is provided in Appendix A. <br /> 2.2 Preparation of the Test Media <br /> Sub-samples of the waste were mixed with the Lab Control/dilution water for 6 hrs,using a <br /> rotary mixer.The sample was a heterogeneous mixture of solid waste that was homogenized as <br /> much as possible before mixing with the Lab Control/dilution water.The Lab Control/dilution <br /> water consisted of synthetic US EPA "soft" water(prepared by adding reagent grade salts to <br /> Type I lab water [reverse-osmosis de-ionized water]).After mixing, these preliminary solutions <br /> were used to prepare test solutions at sample concentrations of 250,500, and 750 mg/L. <br /> 23 Acute Toxicity Testing with Fathead Minnows <br /> The fathead minnows used in this test were obtained from a commercial supplier (Thomas Fish <br /> Company,Anderson,CA). These fish were maintained at 20°C in aerated aquaria containing <br /> EPA synthetic soft water prior to their use in this test. <br /> This hazardous waste evaluation was run at sample concentrations of 250, 500,and 750 mg/L, <br /> with a Lab Control consisting of the same synthetic EPA "soft" water.There were 2 replicates at <br /> each test treatment,each replicate consisting of 9.2 L of media in a 10-L container. Water quality <br /> Pae 1 ( �/ <br /> 4/13 l <br />