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• In addition, the DO level in two select observation wells will be recorded over time following the initial measurement after air injection is terminated. DO levels are expected to decrease over time as the oxygen is being consumed by the indigenous microbes as part of the aerobic biodegradation process. These data will be used to determine the rate of aerobic biodegradation within the treatment area. Initially, the DO levels will be measured at the following frequency: • Once every 15 minutes for the first hour after the initial measurement • Once every 30 minutes for the next 3 hours • Once every hour thereafter for next 4 hours • At least once a day thereafter for next 4 days The monitoring will be completed 5 days after the first post-test measurement or as soon as the DO level reaches to within 10 percent of the baseline condition, whichever occurs first. 4.2.2 Sampling and Analysis Groundwater samples will be submitted for the following laboratory analysis during baseline measurements: • Ammonia nitrogen (U.S. Environmental Protection Agency [EPA] 350.2 or Standard Method [SM] 4500) • Nitrate -nitrite nitrogen (EPA 300.0/353.3 or SM 4500) • Orthophosphate (EPA 365.1 or SM 4500-P) • Heterotrophic plate count (EPA 9215) • Biological oxygen demand (SM 5210-13) • Chemical oxygen demand (EPA 410.3/410.4 or SM 5220 -C/ -D) These samples will be collected from MW-4RS, MW -7S, and VW -21). Results will be used to help determine the amount of oxygen required to promote in-situ aerobic biodegradation at the site and the need for nutrient addition. BiospargePilot Test Final doc 4-4 Shaw Environmental, Inc.