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IRAN -M <br /> growth of gasoline degrading microbes at the Lodi site. The <br /> preferred form of nitrogen is ammonf,um chloride and a balanced <br /> mixture of monobasic and dibasic phosphate provides the needed <br /> phosphorous. Once the limiting nutrients had been identified, <br /> their concentrations were optimized using subsurface soils from <br /> the site so that the microbial metabolic rates are increased with <br /> only modest increases in the total microbial population. It is <br /> essential that the organisms are not allowed unlimited growth. <br /> because this could lead to plugging of the formation (15) with <br /> I microbial biomass and extracellular products. <br /> r <br /> j <br /> f. <br /> Optimization of these nutrients has led to-.an, tial calculation <br /> I that phosphate and ammonia at final concen'tr6tioof 80mg/L <br /> f <br /> phosphate and 100 mg Nil <br /> 4C1/L will be ., qu d (Appendix B) , <br /> k although initial addition levels may lee times that to speed <br /> } saturation of adsorption sit .. f h nutrients. Monitori;ig <br /> will allow us to fine tune t concentrations. Chemical <br /> analyses of polluted wa sa les from June 1987 indicated no <br /> phosphate and extremely to evels of nitrate or ammonium ion are <br /> available to the microbes , Table 4. <br /> SOIL COMPATIBILITY; <br /> J <br /> Soil materials from boreholes Moi-19 and B-1 have been tested to <br /> determine the chemical peroxide. utilization rate .of the soils. <br /> The ,results of those soil .tests show that the subsurface material <br /> :y at Lodi has a moderate reactivity with peroxide, that the <br /> phosphate we, ar.e :planning :to add will help to stabilize the <br /> peroxide, and there was no noticable precipitation in the soil . <br /> slurries due to the addition of the nutrients and peroxide. <br /> f i <br /> Y z <br /> .-25- <br /> n <br /> -25 <br /> •ter <br /> t r <br /> A <br /> h <br /> - <br /> �yN <br /> i <br />