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Pacific EcoRisk Environmental Consulting &Testing <br /> characteristics (pH and dissolved oxygen ID.O.1)were determined for the test solution in each <br /> test replicate prior to initiation of this test.There were 2 replicates at each test treatment,each <br /> replicate consisting of 9.2 L of media in a 10-L plastic container.The test was initiated with the <br /> random allocation of 10 juvenile fathead minnows into each replicate container. These <br /> containers were placed in a temperature-controlled room at 20°C, under fluorescent lighting on a <br /> 16L:8D photoperiod. Note— An initial test of this sample was started on 6/12/08,and the waste <br /> test solutions exhibited a precipitous drop in D.O.overnight,along with complete mortality of <br /> the test organisms. A re-test of this sample was initiated on 6/24/08,and the test solutions were <br /> aerated throughout the test duration. <br /> Each day,each replicate container was examined,and the number of live fish in each was <br /> recorded. Routine water quality characteristics (temperature, pH,and D.O.) of the treatment <br /> waters were measured and recorded for each replicate container each day. <br /> After 96(±2) hrs,the test was terminated and the number of live fish in each replicate was <br /> determined.The fish in a randomly selected Lab Control treatment replicate were then <br /> euthanized and measured for length (to the nearest 0.1 mm) and weighed (to the nearest 0.01 g). <br /> The resulting survival data for the waste treatments were evaluated to determine the LCso using <br /> the CETIS'statistical software (Version 1 .6.5A,TidePool Scientific, McKinleyville, CA). <br /> 3. RESULTS <br /> 3.1 Effects of the Waste Sample on Fathead Minnows <br /> The results of this test are summarized in Table 1.There was 95% survival at the Lab Control <br /> treatment; there was complete mortality in each of the waste treatment levels.These results <br /> indicate that the LCso of this sample is less than the"hazardous waste" toxicity threshold of 500 <br /> mg/L,and that this sample should be considered "hazardous" with respect to fish toxicity. <br /> The test data and summary of statistics for this test are presented in Appendix B. <br /> Table 1. Results of the hazardous waste toxicity evaluation of the waste sample <br /> "3133 AD Art Rd.#two 2". <br /> Sample Treatment Mean % Survival <br /> Lab Control 95 <br /> 250 mg/L 0* <br /> 500 mg/L 0* <br /> 750 mg/L 0* <br /> LCso =>500 mg/L <br /> *Significantly less than the Control treatment response at p <0.05. <br /> Page 2 Pacific EcoRisk L��� <br />