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BIO NATIONAL CORPORATIOI i <br /> Bakersfield, California <br /> GENERAL PARAMETERS FOR BIOREMEDIATION <br /> r <br /> The development of bioremediation technology includes understanding the range of <br /> application and the physical parameters the microbiologist looks for. The physical <br /> environment should meet the following criteria If adjustments can bring the <br /> environment into line economically, then bioremediation is usually the method of ' <br /> choice. <br /> TREATMENT PARAMETERS <br /> 'i <br /> OXYGEN LEVELS - Oxygen pathways are important to initiate hydrocarbon <br /> biodegradation. Aeration, in a reasonably friable soil, can extend naturally a, <br /> as much as three feet. Waterlogging or compaction can reduce the total <br /> amount of air filled pore space. <br /> Aeration is commonly maintained by blower or tilling. <br /> i <br /> I � <br /> COMPACTION - Highly compacted clayey soils are', difficult to bioremediate because <br /> of the inability to produce a free flow of 'oxygen. Sandy or gravelly soils <br /> are ideal for the influx of air and inoculum.' <br /> It is possible to mix the material with more I'f�riable agents. <br /> MOISTURE - Soil moisture levels in the 20 'to 80% range are suitable for <br /> biodegradation. <br /> Soil moisture adjustments are routine in bioremediation processes. <br /> NUTRIENTS - High concentrations of . hydrocarbons in soil, often create a <br /> nutritional imbalance for the microbes assigned to the bioremediation job. <br /> Nitrogen and phosphorus bearing materials may be applied to restore <br /> nutritional balance to the system to be bioremediated. <br /> TEMPERATURE - Bioremediation is practical from 10 to 550 C. (50 to 1320 F.) <br /> Temperatures- below-the=-norm -slow- the process,-and--above--may-destroy -the <br /> microbes. (See Material Safety Data Sheet) <br /> Temperature adjustments can be made: in a controlled remediation <br /> environment. <br /> j: <br /> PH RANGE - Biodegradation is operable in the 5.S to 8.5 pH range, with the ideal <br /> range at 7.0 - 7.5. <br /> pH adjustments are routine in bioremediation processes. <br /> NOTE: As a normal part of the Bio National treatment program, we incorporate <br /> mineral and nutritional supplementation to increase the microbial degradation 1 <br /> of the contaminated material. These vary :.according to the results of our <br /> bio-analysis of representative materials. <br /> y <br /> (1) PARAMTRS <br /> l <br />