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11 <br /> AB Appli <br /> Inc . <br /> • <br /> Bacteriology Lab Report <br /> Sept. 19, 1994 <br /> Attn Bob Marty <br /> Project Geweke Ford <br /> Sample Date I CFU/g eater No Species M ENDO P <br /> TSA of Agar LES Aeroginosa <br /> for Hydrocarbon specific on <br /> Total Bacteria Degraders for MacConkey <br /> Present Coliforms <br /> i <br /> NnV-14 9/19/94 5 x 10' Nonc None � <br /> EW-I 9/19/94 500 S Nonc ;gone j <br /> MVV_S 9/19/94 1 X 10' 5 None Drone <br /> NI-W-11 9/19/94 I 1 -, 10' 4 None None <br /> NMI-12 1 9/19/94 1 1 1 10' b None j None j <br /> Notes' Bacterial cell counts as reflected in TSA agar for total bacteria show an increase <br /> over background of both total cells present and number and types of species present in all Ni ells <br /> This increase is due to the inoculation Several species and strains of hydrocarbon degraders <br /> from the inoculum are present and active These are primarily the species of Pseudomonas <br /> which are all gram negative To a much lesser extent Achromobacter and Streptomyces are also <br /> present Traditionally the gram negative pseudomonads are the primary species to initiate the <br /> degradation process when TPH levels are high This is true of water and soil As TPH <br /> decreases the Achromobacter and the grans positive species such as the Bacillus should appear <br /> and then become dotrunant Based on the current levels of TPH in the capillary fringe and <br /> ground water the bacteriological results are excellent and as predicted and expected The <br /> hydrocarbon degraders are present in moderate numbers matching the moderate contamination <br /> levels The number of hydrocarbon degraders is directly proportional to the level of <br /> hydrocarbon contamination Their numbers are food limited No coliform bacteria or P <br /> aeroginosa were detected in any of the samples Background bacteria (not present in inoculum) <br /> are present in both samples in the same low numbers as prior to inoculation <br />