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16 August 2001 <br /> w <br /> AGE-NC Project No 97-0312 , <br /> Page 16 of 20 <br /> Typically,the microbes utilized in bioremediation of ground water are aerobic and commonly require <br />'* additional oxygen to supplement dissolved oxygen concentrations in ground water Generally, <br /> 'several air sparging or in-well aeration,sources are sufficient to provide the necessary oxygen, t <br />' however, in some cases the addition of hydrogen beroxide or other oxygen source is necessary <br />' 8 2 2 Monitoring Activities <br /> r, <br /> After Inoculation,the inoculation wells and ground water monitoring wells should be monitored for <br />' several months by periodic field measurements of organic vapor,carbon dioxide and oxygen Ground <br /> water samples should be collected and analyzed for petroleum hydrocarbons,bacterial populations <br /> and nutrient availability If monitoring indicates areas of slow remediation, additional Inoculations <br />' may be required <br />' 8 2 3 , Feasibility <br /> Results of bioremediation pilot tests at other sites indicate that lighter petroleum hydrocarbons, such <br />' as gasoline compounds are biodegraded more readily than longer-chain(heavier)hydrocarbons,such <br /> as diesel fuel compounds Feasibility studies have not been performed at the subject site <br /> - ,82-4 Duration-Duration (and cost) of in-situ bioremediation are dependent on the volume of soil and water to be <br /> remediated, the number of inoculation and monitoring wells, the average concentrations of <br />' contaminants and nutrients, temperature and the permeability of soil Theoretically, the radius of <br /> influence of the inoculation well should increase rapidly at first, then gradually decline as the <br /> affected volume increases and the bacteria encounter decreasing hydrocarbon concentrations toward <br />' the margins of the hydrocarbon plume Shorter-chain hydrocarbons are typically consumed first and <br /> longer-chain hydrocarbons later The duration for successful' in-situ bioremediation cannot be <br /> , <br /> predicted because many variables are not' <br /> known <br /> A <br /> ' 8 2 5 Cost <br /> Bioremediation of ground water can be a very cost-effective method of treatment With little on-site <br /> equipment required beyond the initial inoculation,the start upcosts for in-situ bioremediation at the <br />' site would be between$40,000 00 and$60,000 00 However;monitoring will vary depending upon <br /> • regulatory requirements, which could significantly increase the total cost of the project <br />' Advanced GcoEnvironmental,Inc <br />