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� -110culture <br /> ENV IRON MENTAL <br /> B I O T E C H N O L O G Y <br /> CytoCulture International, Inc. 1986 <br /> May 26, 1998 <br /> Client: Upgradient Environmental Consultants <br /> 336 Augusta Ct. <br /> Valley Springs. CA 95252 Project Description: Kwikee <br /> Project No. 98017 <br /> Contact: Victor B. Cherven, Ph.D. Tel: 209-772-3620 Fax: 209-772-3620 <br /> SAMPLES: Three soil samples delivered on ice on 5115198. Samples were stored at 4 Deg. C. until assayed. <br /> Hydrocarbon-Degrading Bacteria Enumeration Assays <br /> .,ANALYSIS REQUEST: Bacterial enumeration for total petroleum hydrocarbon-degraders (broad range <br /> petroleum hydrocarbons: diesel and jet fuel). <br /> CARBON SOURCE: Petroleum hydrocarbons were added as the sole carbon and energy sources for the <br /> growth of hydrocarbon-degrading aerobic bacteria on agar plates. Diesel (Chevron <br /> #2) and jet fuel (1P-4) were blended in a 50:50 ratio and dissolved into the agar to <br /> provide aliphatic and aromatic hydrocarbons in the growth matrix. <br /> PROTOCOL: <br /> Hydrocarbon Degraders: Sterile agar plates (100 x 15 mm) were prepared with <br /> minimal salts medium at pH 6.8 with 1.5% noble agar, without any other carbon <br /> sources or nutrients added. Plates were inoculated with 1.0 ml of sample or a log <br /> dilution of each water sample. Triplicate plates were inoculated with sample log <br /> dilutions of 100, 10-', 10-2 and 10-3. The hydrocarbon plates were poured on <br /> 5118198 and counted after 8 days on 5126/98. The plate count data are reported as <br /> colony farming units (cfu) per gram (g) of sample. Each bacteria population <br /> value represents a statistical average of the plate count data obtained with <br /> inoculations for two of the three log dilutions tested. <br /> Vox 510233.0102 Fax 510.233.3777 Email: Cyto®CytoCulture.com <br /> 249 Tewksbury Avenue Point Richmond California 94801.3829 USA <br />