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A. Keith Kaufman, M.S. <br /> Consultant in Environmental Biotechnology <br /> Serving Industry,Government,Academia and the Legal Profession <br /> January 16, 2001 <br /> TO Mr Tim Cuellar (Advanced GeoEnvironmental, Inc 1 <br /> FROM A Keith Kaufman <br /> Subject Results of Physicochemical and Microbiological Analyses of One (1) Soil <br /> Sample (Pacific Pride/El Dorado) <br /> Listed below are the results from the referenced analyses performed on one (1) soil sample <br /> collected on 12/04/00 and received on 12/27/00 After appropriate dilution, samples were <br /> plated onto Trypticase Soy Agar (TSA, for general microbial enumeration) and Minimal Salts <br /> ' Agar (MSA) supplemented with 2500 ppm gasoline as sole carbon substrate Media were <br /> prepared at ambient pH Cultures were incubated under aerobic conditions at room <br /> temperature for six (6) days prior to enumeration Physicochemical analyses were performed <br /> ' utilizing the HACH Spectrophotometric System in accordance with manufacturer's <br /> specifications <br /> RESULTS <br /> Ij <br /> o <br /> �= �ararrt@teC €s tt{ r Fz <br /> &a <br /> -PH' <br /> pH' � s1, _ 7 t„"_tG6 <br /> Nitrate- ppm)N1, <br /> W8P <br /> A` � t F �," v. � x�-s� m <br /> x} Po Nitrate (pPri�}'ff, na � a 4 s �; 2 <br /> =Ahnmonia-N,(ppm) <br /> 'Al <br /> Y a r q„sk hz X7, m� - x aA x r `y x u! ?,1"� r T r s <br /> g 'st <br /> € i "{�YthQl?h}�o"sphate (ppml � m t' r€"``3 4U � <br /> "y <br /> ;; a< �a ,"W ,r N�`� <br /> i, ✓ � �"� ��% e `�" ='� � `�� �'s� � �,«?4 ",�s� y. :m��� <br /> ' y°r Gen Micr'-tEnum A- n�a E� ' a, <br /> rf t - <br /> u 3„ <br /> � � ui <br /> z, � „r " �� r - <br /> i Sel�IVIICr. �ntJr�t ,��.r ��,���^� ,a �r,r* a '' m� � I S'",=�''y <br /> � '� <br /> "(CFU.X v 1041 .:^toe^-" -y'a "`' ��Lj. y ,.. t� a ,fie" <br /> b*, <br /> M1„' "an iq€. x NSR <br /> ¢ .2 "1s�^vYtF <br /> xty,­;ii '" �t <br /> zs <br /> v <y a i sfY �zx" ate, i8 e ,i sa y <br /> ."�r' ,� � aeras � �• � r� ���- '�^.o�q � <br /> xQ�c Bii ddgraderS ' "�§� , =sem 4 k�Q�l) <br /> ' ND, Not detected at limit of 0 1 ppm 1 102 dilution <br /> CFU, Colony-forming Units (viable cells) <br /> United States Office Asia-Pacific Office <br /> 6245 Bristol Parkway#249 2nd Floor#162 Tung-An Strut,Ta-An District <br /> ' Culver City CA 90230 Taipei,Taiwan R 0 C <br /> (310)822-9385 (310)577-9364[FAX) 886-2-378-3751 886-2 378-3752[FAX) <br />