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Steve Royall NCPA C3-2 Discharge Characterization Page 2 of 6 January 22, 2007 Sampling Program In accordance with the Regional Board request, NCPA requested FGL Environmental, an FLAP certified laboratory, to analyze both the water received from the White Slough WPCF(influent) and the effluent returned to the White Slough WPCF(effluent). After a review of the results from the first set:of sampling events, it was decided to re-sample for five parameters because of laboratory QA/QC issues. Initial lab results from FGL for sulfite and endrin carried QA/QC flags which indicated that the laboratory did not feel the reported results were reliable ("did not conform"). Dichloromethane (methylene chloride) was not detected in the influent, but was detected in the effluent; because dichloromethane is known to be a common lab contaminant it was selected for re-test. Pentachlorophenol was not detected in the influent, but was measured in the effluent at only 0.1 uglL over the PQL. The re-test results for sulfite by EPA 377.1, endrin by EPA 3520C18081, methylene chloride by EPA 5030B18264B,and pentachlorophenol by EPA625 all were reported as non-detect at the detection levels noted in Table 1. Initial copper results also appeared questionable, in light of the fact that measurements of metals in high salinity water by standard methods frequently overstate metals concentrations. To gain a more reliable measurement of copper in the influent and effluent, re-test samples were sent to Frontier Geosciences in Seattle Washington. Frontier has developed widely accepted methods for the preparation of high salinity samples for analysis of metals by ICP-MS. The Frontier results are reported in Table 1. This report is based on the following lab reports provided by FGL Environmental, Stockton, California: 6361 1 1, 636407, 636525,636556,636573,636872,636873, 638254, 650014, and 650133. A tabular summary of all of the analytical data contained within these lab results is attached as Table 1, and summarized in this document. A summary of the bioassay results is included below, in Table 2. Bioassay Summary: Table 2 below shows the results of short term chronic toxicity testing performed according to the guidelines prescribed in EPA-821-R-02-013,Short Tenn Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. In Table 2, "inf'denotes to NCPA influent (WPCA effluent),and"eff' denotes NCPA effluent. Table 2—Three Species Bioassay Results Summary NOEC inf NOEC eff IC25 inf IC25 eff IC50 inf IC50 eff Minnow—survival 50.00% 12.50% X100% 20.24% X100% 40.63% Minnow —growth 12.50% 25.00% 59.90% 25.18% >100% 41.46% Water flea— survival 100% 50.00% X100% 58.33% X100% 72.22% Water flea— 100% 25% X100% 35.38% >I009c 51.82% reproduction Algae -growth 25.00% 100% >100% >l[)0cic >100% >100%