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BES <br />1. INTRODUCTION <br />The California Department of Substances Control has adopted regulations (R-45-78) which <br />define criteria for the identification of hazardous wastes. These criteria are codified in Chapter <br />11, Article 3 of Title 22 of the California Code of Regulations. Toxicity to aquatic life, <br />specifically fish, is one of the criteria used to gauge the hazardous potential of a waste. An acute <br />96 -hour bioassay is used to determine the LC50 as defined in Section 66261.24(a)(6) of these <br />regulations. This 96 -hour LC50 value serves as the numerical indicator of the toxicity of a waste <br />to aquatic life. The sample is deemed hazardous if the LC50 is less than 500 mg1L. <br />This report describes the procedures used and the results obtained for the hazardous waste <br />aquatic toxicity screening test(s) performed by Block Environmental Services (BES) for <br />California Laboratory Services. <br />BES is an Environmental Laboratory Accreditation Program certified laboratory (#1 812). <br />2.1 TEST ORGANISMS <br />• Fathead Minnow, Pimephales promelas, obtained from a commercial supplier or.in- <br />house culture. <br />2.2 TEST PROCEDURES <br />A detailed procedure for this test is outlined in DPHS-ELAP approved standard operating <br />procedures (SOPS), which are on file at the BES laboratory. These SOPS are based upon the <br />following references: <br />• California's Title 22 Code, Section #66261.24(x)(6); Static Acute Bioassay <br />Procedures for Hazardous Waste Samples, Polisini and Miller, 1988, California <br />Department of Fish and Game <br />• Guidelines for Performing Static Acute Toxicity Fish Bioassays in Municipal and <br />Industrial Wastewaters, Kopperdahl, 1976, California Department of Fish and Game <br />• Standard Methods for the Examination of Water and Wastewater, 19th Edition, <br />American Public Health Association, 1995. <br />BES #24548 Page 2 of 3 <br />