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r � <br /> of heterotrophic bacteria which typically can lead to the inhibition of growth in the selective <br /> contaminant-degrading populations via competition for available inorganic nutrients. This factor <br /> alone can often dictate the feasibility and appropriateness of a biostimulatory or bioaugmentation <br /> approach. <br /> In addition to providing valuable information from a microbiological perspective, TOC analyses <br /> are also considered routine for NA or RBCA approaches by providing information pertaining to <br /> the sorption potential of chemical compounds and migratory activity (retardation), as well as <br /> providing indications of potential chemical desorption (concentration spikes), for example as a <br /> result of oxygen addition, as biological activity progresses. <br /> Iron II - Fe+z (Dissolved Iron, in situ only): The routine analysis of Iron (Il) provides initial <br /> information in detecting the development of precipitates (scaling) which could restrict intrinsic <br /> permeability and indications of the initial feasibility of transporting essential biological components <br /> (e.g., moisture, nutrients, oxygen, terminal electron acceptors, etc.). In addition, this analysis can <br /> commonly be utilized as a marker for indications of naturally-occurring intrinsic bioremediation <br /> (generally via anaerobic developments). <br /> In addition to conducting these baseline laboratory analyses, it is recommended that field personnel con- <br /> duct field analyses consisting of temperature, dissolved oxv_,gen, and redox potential measurements when <br /> collecting samples (please inquire with BioLogic for procedures and appropriate sampling techniques). <br /> EVALUATIONS OF THE EFFECTS OF PHYSICOCHEMICAL PARAMETERS <br /> Subsequent to obtaining baseline verification of microbial viability (via above testing), the following tests <br /> have primarily been designed for those desiring to pursue any form of indigenous applications. Whether the <br /> desired approach is to just let it passively sit in a stagnant pile or more active biostimulation, via nutrient, <br /> moisture, oxygen additions, tilling etc., the tests can cost-effectively address at least fundamental contribut- <br /> ing or limiting factors which may influence a selected approach, without incurring the time and expense of <br /> full-scale treatability studies. These simple evaluations can accommodate those choosing to minimize <br /> bioremedial/project expense, and/or who may automatically presume that ubiquitous bacteria will degrade <br /> a contaminant, by providing valuable information in advance as to whether and what degree microbial <br /> activity may/can occur, and in determining the appropriateness or operational requirements necessary for a <br /> selected approach in order to avoid project failure. <br /> In cases where baseline physicochemical and microbiological analyses produce less than desirable results <br /> for effecting site cleanup and/or indicate environmental incompatibility as demonstrated, for example, by <br /> low heterotrophic and selective population numbers, the issue of possible physicochemical limiting factors <br /> might be addressed. Low recoveries of microbial populations can be attributed to one or more specific <br /> growth-limiting conditions which tend to be site-specific and may include: environmental parameters such <br /> as nutrient concentrations, pH, temperature, oxygen, contaminant levels, and salinity. These tests, as <br /> described in the following sections, have been designed to evaluate the effects of individual parameters on <br /> the growth of indigenous heterotrophic and selective microbial populations in an effort to determine which, <br /> if any, of the factors may be limiting to microbial growth and biodegradation efficiencies. Each of the <br /> following evaluations is designed to be performed subsequent to obtaining baseline microbiological and <br /> physicochemical results, and will consist only of those evaluations of potential inhibiting factors as identi- <br /> fied from background analyses. Additionally, provided arrangements are made in advance, these tests can <br /> be performed on samples collected initially for background analyses. This can eliminate additional remo- <br /> bilization costs which would be incurred if further sampling were required in order to obtain the necessary <br /> supplemental data. <br /> 5 <br />