Laserfiche WebLink
glove box The plate count data are reported as colony forming units (cfu) per milliliter (ml) of <br /> sample Each enumeration value represents a statistical average of the plate count data obtained <br /> from two of the four inoculating log dilutions assayed <br /> Protocol for Anaerobic Hydrocarbon-Degrading Bacteria: Pasteurized Chevron diesel No 2 <br /> and regular gasoline were dissolved into agar plates as the sole carbon and energy sources for the <br /> growth of anaerobic hydrocarbon-degrading bacteria The medium includes alternative terminal <br /> electron acceptors such as sulfate,nitrate, and iron Sterile agar plates (100 x 15 mm) were <br /> prepared with minimal salts medium at pH 6 8 with agar and hydrocarbons, without any other <br /> carbon sources or nutrients added Plates were set up and poured in a Coy anaerobic glove box <br /> under strict anaerobic conditions (atmosphere of nitrogen, carbon dioxide and hydrogen) <br /> Triplicate plates were inoculated with 1 0 ml of each sample (log dilution 10°) or log dilutions of <br /> the sample at 10-r, 10-Z, and 10-3 Hydrocarbon plates were counted after 13 days incubation <br /> inside the glove box at ambient temperature The plate count data is reported as colony forming <br /> units (cfu) per milliliter (ml) Each enumeration value represents a statistical average of the plate <br /> count data obtained from two of the four inoculating log dilutions assayed <br /> ANAEROBIC <br /> Hydrocarbon-Degrading and Total Heterotrophic <br /> Bacteria Enumeration Assays <br /> Client Sample Sample Hydrocarbon Target Total <br /> Degraders Hydrocarbons Heterotrophs <br /> Number Date cfu/ml) Tested (cfu/ml) <br /> MW-1 12/16/03 4 x 103 Gasoline/Diesel 1 x 104 <br /> MW-4 12/16/03 3 x 102 Gasoline/Diesel 5x 104 <br /> MW-5B 12/16/03 1 x 103 Gasoline/Diesel 3 x 104 <br /> MW-8 12/16/03 2 x 142 Gasoline/Diesel 3 x 102 <br /> Sterile Water 12/17/03 0 Gasoline/Diesel 0 <br /> Air Control 12/17/03 0 Gasoline/Diesel 0 <br /> Positive Control 12/17/03 4x 10$ Gasoline/Diesel 8x 10$ <br /> Reporting Limit for enumeration data is l 0 x 10 cfu/ml <br /> A positive control sample of hydrocarbon-degrading bacteria was run concurrently with these <br /> samples using a mixed flask culture of bacteria isolated from contaminated groundwater sites to <br /> Northern California <br /> 3 <br />