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ANAEROBIC Hydrocarbon-Degrading Bacteria Enumeration Assay Results Client Sample Sample Hydrocarbon Target Total Number Date Degraders Hydrocarbons Heterotrophs (cfu/ml) Tested (cfu/ml) MW-1 03/05/03 6 5 x 10 z Gasoline/Diesel 1 x 103 MW-4 03/05103 3 x 10 Gasoline/Diesel 2 X 104 MW-SB 03/05/03 8 x 10 3 Gasoline/Diesel 1 X-10 4 Sterile Water 03/05/03 Zero Gasoline/Diesel Zero Air control 03/06/03 Zero Gasolme/Diesel Zero Positive Control 03/05/03 3 x 10 6 Gasoline/Diesel 6 5 x 107 Reporting Lima for enumeration data is 1 0 x 10 1 cfulml A positive control of anaerobic hydrocarbon-degrading bacteria was run concurrently with these samples using a mixed flask culture of anaerobic bacteria isolated from contaminated groundwater sites in Northern California Inorganic Chemistry and Nutrient Assays Analysis Request: Nutrient assays were preformed for pH, dissolved oxygen (DO), oxidation-reduction potential (ORP), ammonia nitrogen, ortho-phosphate, sulfate, nitrate, and ferrous iron Protocol- Spectrophotometric assays were performed to determine the concentrations of ammonia, ortho-phosphate, sulfate and nitrate The assays follow EPA manual colorimetric protocols using precalibrated reagents and a Bausch & Lomb spectrophotometer 20 All assays conform to California CLP and Standard Water & Wastewater Methods The testing for ferrous iron and alkalinity was subcontracted out to Curtis & Tompkins, Ltd Analytical Laboratory ortho- Nitrate Client Ammonia Phosphate Nitrogen Sulfate Sample ORP mg/L mg/L mg/L mg/L Ferrous Iron Number pH DO (mV) (ppm) (ppm) (ppm) (Ppm) mg/L (ppm) MW-1 607 590 214 067 280 084 6200 0 13 MW-4 639 370 224 077 171 059 6200 <0 10 MW-5B 653 480 1 188 057 054 . 033 500 1 60 Reporting limit for the colorimetric chemistry assays is 0 1 mg/L Reporting limit for ferrous iron is 0 l0 mg/L Reporting limit for alkalinity is 1 0 mg/L