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' y <br /> BioJet <br /> novatllve Remediation Technologies <br /> Mr Bnan Millman Project Name Grantlme Gas and Food Sampled 09/17/01 <br /> Advanced GeoEnvironmental,Inc Client Project ID Received 09/19/01 <br /> 837 Shaw Road Sampled By Plated 09/20/01 <br /> Stockton,CA 95215 Analysis Run Microbial&Physicochemical Enumerations Analyzed(Physio) 09/20/01 <br /> Phone 209-467-1006 Fax 209-467-1118 Laboratory Indentification BJ-AGE-GR-1-2 Enumerated 09/26/01 <br /> P O # Plating Concentration 100 ppm Gas 1165 ppm Diesel Reported 09/27/01 <br />' Site Location SioJet Pro ect Manager Ken Farrar 269-245-6044 Fx 209-245-3765 <br /> Listed below are the results of microbial and physicochemical analyses,performed on two(2)water samples collected on September 17,2001 from the <br /> Grantline Gas&Food site and received by BloJet on September 19,2001 <br /> Samples were analyzed for pH and concentrabons of nitrogen(nitrate, nitnte,ammonia),phosphorus(as ortho-phosphate)and potassium utdong a Hach <br /> DR2000 spectrophotomenter and pH meter in accordance with the manufactures instructions General(heterotrophic nonspecific)and selective(Gas/ <br /> Diesel specific)enumerations were performed,respectrvely an Plate Count Agar(nutritionally complex)and 50%Bushneb-Haas minimal salts media <br /> supplemented with 100 ppm diesel/gas,as the sole carbon source Using standard microbiological plate count techniques,semi dilutions of each water <br /> sample were inoculated onto each media type and incubated,aerobically for six(6)days at 30 degrees Celsius prior to evaluation A single dilution for each <br />' sample were performed in triplicate with an average of all three bang reported for each sample <br /> Laboratory Results <br />' Sample GEN SEL DEG <br /> Sample ID# Descnpton D 0 TOC pH N0,-N NO, NO,N NO, NHrN NH4 PO4 K 1 00E+05 1 00E+05 % <br /> 1 MW-1• 75 4 176 62 2034 1 1 1 419 17 35 61 1 1 1803% <br />'mi MW-4" 77 7 308 6 2624 084 10" 12 32 71 08 11 270/a <br /> NW <br /> Plated using Gasoline as the sole carbon source <br /> Plated using Diesel as the sole carton source <br /> 1 <br /> Signature <br /> Ken F onager <br /> MC= Mashre Corfu(%) NOrN: N*tt1)-Nrir0ge11(Ppm) PO4= OM-Phosoxta(Ppm) <br /> pH=bg Hydrogen on Corx rntrauoo Noz 0 Nihnm(ppm) K=Pohassimn(ppm) GEN=Heterotrogrc Orgarusnq(CPU x 10) <br /> OrN=NrtrahE�N*agan(ppm! NHrN=Amnona Ni rogan(ppm) Detection Limifa for SEL=Seiecbw Degrader Orga--(CFU x 10 <br /> NOS=NitrateIon(ppm) NH,.AmrarwWrI1011(ppm) inorganics.0 01-0 1 ppm %=Percentage of Stied"fleeaderorgamisms <br /> I <br />