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4/2/2019 Effect of C/N Ratio on the Removal of Nitrogen and Microbial Characteristics in the Water Saturated Denitrifying Section of a Two-Stage C... <br /> packing medium vertically, and left by the outlet where the water quality was measured. In order to <br /> regulate the C/N ratio of the influent of the second stage, a tank with a volume of 1 L was installed <br /> between the columns, from which a carbon source could be added. <br /> 11 11 <br /> 13 <br /> 3 104 <br /> eeTef�"�""d 8 <br /> the first stages stem i.. 1 the second stage system <br /> 12 <br /> Figure 3 <br /> Schematic diagram of the simulated two-stage column CRI. 1:Feeding tank(V=5 L);2:Peristaltic pump; <br /> 3:Thermometer;4: Tank to add a carbon source(V= 1 L);5: Temperature controller;6: Filling medium; <br /> 7: Support layer; 8: Valve;9: Saturated section; 10: Insulation mantle; 11: Air hole; 12: Effluent outlet of <br /> the first stage; 13: Effluent outlet of the second stage. <br /> 2.2. Sewage and Operational Conditions <br /> The influent used in this study was synthetic sewage made up of glucose, sodium acetate, ammonium <br /> sulfate,ammonium chloride,potassium phosphate, sodium carbonate and peptone,which was refilled <br /> every three days. The water quality parameters are shown in Table 1. The sewage influent entered the <br /> first stage column via a dry-wet alternating operation mode, with water feeding with a hydraulic load of <br /> 0.6 m3•(M-3-d7l),each feeding time would last for 1.5 h and this was performed twice every 24 h, <br /> followed by a drying time lasting 10.5 h,and a water flow of 200 mL/h. The second-stage column <br /> received the effluent of the first stage column as influent. This was added by intermittent feeding using <br /> the same regime as for the first stage column. <br /> https://www.ncbi.nim.nih.gov/pmc/articles/PMC6069465/ 5/18 <br />