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4/2/2019 Effect of C/N Ratio on the Removal of Nitrogen and Microbial Characteristics in the Water Saturated Denitrifying Section of a Two-Stage C... <br /> Initially, the TN concentrations in the second-stage effluent of all three experiments were above 35 <br /> mg/L during the first 5 days(Figure 5a),which indicates a relatively poor nitrogen removal <br /> performance at this early stage of the experiments. Possibly,the denitrifying bacteria in the second <br /> phase need time to adapt to the new conditions applying after addition of an external carbon source. <br /> From day 9 onwards,the TN concentration in the second stage effluent of T2 and T3 decreased,to 34.2 <br /> mg/L and 21.9 mg/L respectively. As the experiments continued,this TN concentration in effluent of <br /> T3 kept declining,but in T2 it increased again. This indicates that the C/N ratio of 2:1 enhanced the <br /> denitrifying capacity of the second stage section to a certain extent by promoting growth of denitrifying <br /> bacteria,but it was insufficient to meet the increasing demand of carbon as the denitrifying bacteria <br /> proliferated. This is in line with findings reported by Fan et al. [28],who tested a C/N ratio of 2.5 in <br /> influent of a constructed wetland which resulted in a TN removal efficiency of only around 25%. <br /> On day 17 of the experiments, the TN concentration in the second stage effluent of was more or less <br /> stable with mean TN concentrations in the second stage effluent of 48.4 mg/L(giving a 19.6%removal <br /> rate)in Tl,41.0 mg/L(31.8%removal rate)in T2 and 14.7 mg/L(75.4%removal rate)in T3. The TN <br /> removal rate of the second stage effluent had thus nearly doubled in T2 compared to T1,but the <br /> resulting concentration was still too high to meet the national sewage discharge B standard(GB 18918- <br /> 2002)(TN:5 20 mg/L). The second-stage effluent of T3 passed this standard, and even passed the <br /> sewage discharge a standard(TN:5 15 mg/L). The TN removal of T3 had improved by <br /> compared to I. This clearly shows that addition of a carbon source in the second stage influent <br /> enhanced the nitrogen removal efficiency of the system,whereby a C/N ratio of 5 performed better <br /> than a ratio of 2. The best TN performance obtained resulted in"-79.8%removal of TNS <br /> 3.3. Effect of C/N Ratio on Removal Efficiency of Chemical Oxygen Demand <br /> The COD concentration of the first stage influent was 268.3 f 20.0 mg/L. This reduced during the first <br /> stage to 22.8 mg/L, 24.5 mg/L and 20.5 mg/L for effluent of T1,T2 and T3 respectively, so the mean <br /> fraction of removed COD reached up to 90% in all three experiments. However, the mean <br /> concentration of NO3 -N in the first stage effluent remained above 48.3 mg/L, resulting in a C/N ratio <br /> in the first stage effluent below 1. The effect of increasing this ratio to 2 and 5 in T2 and T3, <br /> respectively,on COD removal is shown in Figure 6. Whereas the mean concentration of COD in the <br /> second stage effluent of T 1 decreased to only 16.2 mg/L, for T2 and T3 it went down to 9.3 mg/L and <br /> 8.7 mg/L respectively. Compared with Tl,the mean COD concentration in the second stage effluent of <br /> T2 and T3 were much lower,indicating that the addition of glucose to the second stage influent had not <br /> resulted in an increase of COD in the effluent,but rather promoted the removal of COD during the <br /> second stage. Clearly,this external carbon source enhanced the activity and growth of denitrifying <br /> bacteria. The amount of glucose required for this was not fully met under experimental condition T2,as <br /> the best COD removal was obtained with T3,which received the highest amount of glucose. In another <br /> study,Chen et al. [7] found that the removal efficiency of COD could reach up to 85%in the nitrifying <br /> section of CRI,while Wang et al. [20] determined an optimal C/N ratio for denitrification of 6-7 when <br /> they used glucose as external carbon source. Zhang[29] noticed that nitrogen could be completely <br /> removed in SBR by adjusting the C/N ratio to 7.1 with added glucose. Yan et al. [30] reported that if <br /> the main purpose of the sewage treatment was to remove nitrogen, the C/N ratio in influent should be <br /> slightly lower than the optimum C/N ratio,which could not only guarantee high COD removal rates <br /> (resulting in low residual organic matter),but also produce a better nitrogen removal performance. <br /> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069465/ 11/18 <br />