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1 <br /> cyto <br /> Culture <br />' E N V I R O N M E N T A L <br /> B I O T E C H N O L O G Y <br />' CytoCulture International, Inc 1986 <br /> tMay 1, 1998 <br /> r - <br />' Client: CLS Labs CLS Workorder# P3548 <br /> Contact: George Hampton Tei: 916-638-7301 Fax: 916-638-4510 <br />' SAMPLES: Three soil samples were received on 4/24/98 The sample was stored at 4°C until assayed on <br /> 4/24198 <br />' Hydrocarbon-Degrading Bacteria Enumeration Assays <br /> ANALYSIS REQUEST: Bacterial enumeration for total petroleum hydrocarbon-degraders (broad range <br />' petroleum hydrocarbons diesel and Jet fuel) in soil <br /> CARBON SOURCE Petroleum hydrocarbons were added as the sole carbon and energy sources for the <br />' growth of hydrocarbon-degrading aerobic bacteria on agar plates Diesel (Chevron <br /> #2) and JP-4 Jet Fuel were blended in a-50 50 ratio and dissolved into the agar to <br /> I _ provide aliphatic and aromatic hydrocarbons in the growth matrix <br /> PROTOCOLS <br /> Hydrocarbon Degraders Sterile agar plates (100 x 15 mm) were prepared with <br /> minimal salts medium at pH 6 8 with 1 5%noble agar, without any other carbon <br /> sources or nutrients added Plates were inoculated with 1 0 ml of sample or a log <br /> dilution of each water sample Triplicate plates were inoculated with sample log <br /> dilutions of 100, 10-1, 10-2, l 0-3,and 10-4 The hydrocarbon plates were poured on <br /> 4/24/98 and counted after 7 days on 511198 The plate count data are reported as <br /> colony forming units (cfu)per gram (gm) of sample Each bacteria population <br /> value represents a statistical average of the plate count data obtained with <br /> inoculations for two of the four log dilutions tested <br /> l 4 1 1 v r <br /> - r <br /> 4 <br /> Vox 510 233 0102 Fax 510 233 3777 Email CytoQCytoCulture cam <br /> 249 Tewksbury Avenue Point Richmond California 948013829 USA <br />