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Sent By< MCCampbell Analytical; • 925 798 1622; Feb-7, 23:07; Paye 315 <br /> 1. INTRODUCTION <br /> 'l'lle California Department of Substances Control has adopted regulations (R-45-78)which <br /> define criteria for the identification of hazardous wastes. Thuse criteria are codified in Chapter <br /> 11, Article 3 of Title 22 of the California Code of Regulations. 'Toxicity to aquatic life, <br /> specifically fish, is one of the criteria used to gauge the hazardous potential of a waste. An acute <br /> 96-hour bioassay is used to determine the LC50 as defined in Section 66261.24(a)(6) of these <br /> regulations. This 96-hour LC50 value serves as the numerical indicator of the toxicity of a waste <br /> to aquatic life. The sample is deemed hazardous il'the LC50 is less than 500 mg/L. <br /> This report describes the procedures used and the results obtained for the hazardous waste <br /> aquatic toxicity screening tcst(s) performed by Block Environmental Services (BES) for <br /> McCampbell Analytical (Project #13271-A; Project Name: Jamar Exxon). <br /> RFS is an Environmental Laboratory Accreditation Program certified laboratory (91812). <br /> 2. MATERIALS AND METHODS <br /> 2,1 TEST ORGANISMS <br /> • Fathead Minnow, Pimephales promelas, obtained from a commercial supplier. <br /> 2.2 TEST PROCEDURES <br /> A detailed procedure for this test is outlined in laboratory standard operating procedures (SOPS) <br /> kept at the BES laboratory. These SOPs are based upon the following references: <br /> • California's Title 22 Code, Section #66261.24(a)(6)-, Static Acute Bioassay <br /> ProceduresJor Hazardous Waste Samples, Polisini and Miller, 1988, California <br /> Department of Fish and Game <br /> • Cuidelines•,fvr Performing Static Acute Toxicity fish Bioassays in Municipal and <br /> Industrial Wastewaters, Kopperdahl, 1976, California Department of Fish and Game <br /> • Standard Methods Jnr the Examination of Warer and Wastewater, 19'h Edition, <br /> American Public Health Association, 1995. <br /> 2.3 DATA ANALYSIS <br /> All toxicity testing results will be analyzed using an appropriate statistical method to determine a <br /> LC50 and the corresponding 95% confidence limits. <br />