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' 2.0 Work Plan o a The preferred alternative entails the use of an enzyme catalyzed bioremediation process The enzymatic compounds, amino acids, and biosurfactants to be used as !, part of the remediation have been extracted from live bacteria from the Tuman- Pecora region of Siberia The process was brought to the United States by Emerging ' Technologies International under the trademark ETEC, and has been used throughout the United States Currently, the ETEC" process is being used by Aprotek at the McCormick-Baxter Superfund site under the U S Environmental Protection Agency's(USEPA's) Superfund Innovative Technology Evaluation(SITE) ' Program, for the remediation of creosote and dioxin impacted groundwater 2.1 Technology Overview ' The ETEC`"multi-enzyme process uses enzymes, amino acids, and biosurfactants that are released from bacteria during autolysis Autolysis is a naturally occurring ' process during which self-produced enzymes destroy the bacterial cell wall The ETEC'"multi-enzyme complex(monomethanoxigenase,alcoholo dehydrogenase, and aldehydehydrogenase) is the "active part" of the living bacteria Under the ETEC'" process, the enzymes are separated, stabilized, and concentrated into a crystalline form During application, the enzymes are reconstituted in water, and applied with biosurfactants to the impacted medium The use of biosurfactants enhances the contact time between the enzyme and the contaminant The bioremediation process occurs when the enzymes, serving as organic catalytic agents, stimulate a series of chemical reactions resulting in the oxidation of both ' short and long-chain hydrocarbons into alcohol, aldehyde, and finally,monobasic fatty acids The production of fatty acids help emulsify the hydrocarbons, and the amino acids promote the rapid growth of the natural bacterial population This "biopreparation" process results in the breakdown of the hydrocarbon molecule Once the outer molecular walls of the hydrocarbons are emulsified,the hydrocarbons ' are readily accessible to the natural bacteria The bacteria and enzymes attach to the hydrocarbon forming a transition state complex (emulsification and engulfing of the hydrocarbon by the enzyme bacterial product complex) The end result of the reaction is the complete breakdown of the emulsified hydrocarbon into fatty acids, carbon dioxide, and water The life-span of the enzymes upon infection is two weeks 94118 East Bay Municipal Utility District ell 40429601 rap 6 Remedial Action Plan 1