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M��Laboratories Inc. <br /> Environmental Testing Laboratory Since 1949 <br /> 6 <br /> Subcontract Report for 1632908 PDF File Name: wo_1632908_sub_all.pdf Page 3 of 4 <br /> FATHEAD MINNOW HAZARDOUS WASTE <br /> SCREEN BIOASSAY pri Aquatic <br /> Testing <br /> Lab No.:_ At S f! 1 4o I–po 2 QC <br /> Laboratories <br /> Client/ID: 13 L 19 32 q u 2 –o 3 .4 <br /> TEST SUMENIARY <br /> Species:Pimephates promelas. Source:Thomas Fish. <br /> Fish weight(gm):av: 0j-;titin:O a! ,max: tit r'S Regulations:CCR Title 22. <br /> Reference Toxicant:SDS conducted per batch. Test Protocol:California F&G/DHS 1988. <br /> Test chamber volume: 10 liters. Endpoints:Survival at 96 hrs. <br /> Temperature:20+/-2°C. Test type:Static. <br /> Aeration:none,unless D,O.drops below 5.0 mg/I. Feeding:None. <br /> Number of replicates:2. Number of fish per chamber: 10. <br /> Dilution water:Soft reconstituted water(40-48 mg/I CaCO3). Photoperiod: I6/8 hrs light/dark. <br /> TEST DATA <br /> INITIAL 24 Hr 48 Hr 72 Hr 96 Hr <br /> Dalelrime: too !o o tz-)-f I (olo -t I fo a <br /> Analyst: <br /> °C DO pH °C DO pH tl D °C DO pH q D °C DO pH b D °C DO pH #D <br /> Control A L- s% R.o O g_ O t7 � _d Jt.B q 4_ <br /> Control 8 4L Q. -v o.I S. 3 �• 0 2J. i g, 4 g.6 O 8- S i, ( (7 S. 6 S.0 G <br /> 400mg/IA ,7 g,) S•0 0.1- 4r°I 7 20. f.y 7. 6 20. 6.a D 20. 8. Sa <br /> 400mg/IB I _p b• B•o 7. O ). D g,o Z 9.-C )q <br /> 750 mg/I A Q, ) $,v )_ Q 2•. 7. O a_ Pit 7.`I O & ) O <br /> 750--n B t,Y Sr 9 9,0 70 4. 2o.1 r. .% C, 2 1,1 7.8 O <br /> Comments: Extraction method:Mechanical shaking t/ . <br /> None(aqueous solution) .,4 <br /> Dissolved Oxygen(DO)readings in mg/I O,. Test Aerated: / No <br /> CONTROL HIGH CONCENTRATION "Total Number Dead <br /> Alkalinity Hardness Alkalinity Hardness Control O /20 <br /> Initial 12 mitt,CaCO, y� mF/I CeCO, 3 me Cern, me caco, 400 mg/I � /20 <br /> Final mitt,C-co, tI G mitt,Cisco 3 2 me Caco, mg/l CAM, 750 m l � /20 <br /> RESULTS <br /> the checked ✓ result applies based on fish survival rates of this test; NA-notapplicable) <br /> PASSED LC50>750 mg/l (<40%dead in 750 mg/1 cone,) <br /> -1q FAILED X40%dead in 750 mg/I (close to passing-definitive test recommended) <br /> FAILED LC50<400 mg/1 (>60%dead in 400 mg/I cone.) <br /> The results in this report apply to the samples onaly_ed in accordance with the chain of custody document.This analytical report must be reproduced to its entirery <br /> All results listed in this repcn are for the—lwiv,use of the submmmg party aC L boratane,Inc assumes no respotmib,bty for report al;ention,separation,detachment or thud parry inlerpretanan <br /> 4100 Atlas Court Bakersfield,CA 93305 (661)327-4911 FAX(661)327-1918 www.bciabs.com Page 41 of 43 <br />